Unit:
Κατεύθυνση Έρευνα στην Γυναικεία ΑναπαραγωγήLibrary of the School of Health Sciences
Author:
Γρηγοριάδης Χαράλαμπος
Supervisors info:
Αν. Καθηγήτρια Aγάθη Κόνδη-Παφίτη (επιβλέπουσα) , Καθηγητής Γεώργιος Κρεατσάς , Διδάκτωρ Γεώργιος Βαλσαμάκης
Original Title:
Kύτταρα Hofbauer.Ανοσοϊστοχημική μελέτη της κατανομής και της μεταβολής αυτών σε φυσιολογικούς και παθολογικούς πλακούντες δια των δεικτών CD1,CD14,CD68,IFN-α,IL-1,Ki-67.
Summary:
Hofbauer cells (H.c.) are fetal tissue macrophages of the human placenta. Ιn
placentas from uncomplicated pregnancies, H.c. either disappear or become
scanty after the 4th to 5th month of gestation.
Totally, 70 placenta's specimens were examined. i) placenta's from normal
uncomplicated pregnancies (16), ii) placenta's from first trimester's
miscarriages (10), iii) placenta's from cases with prenatal diagnosis of
chromosomal abnormality (26) between the 13th and the 23rd week of gestation,
iv) placenta's from pregnancies complicated with intrauterine growth
restriction (IUGR) (8), and v) placenta's from pregnancies complicated with
gestational diabetes mellitus (10).
Histological study of Hematoxylin - Eosin sections was performed while
immunohistochemical study followed by using the markers: CD14, CD68, Lysozyme,
A1 Antichymotrypsine, CK7, Vimentin, IL-1, Ki-67.
In normal term pregnancies, H - E study showed the presence of H.c. in 37.5% of
cases. However, immunohistochemical study using A1 Antichymotrypsine revealed
their presence in 87.5% of cases.
In first trimester's miscarriages, basic H - E study and immunohistochemistry
were positive for H.c. presence in all cases.
In cases with prenatal diagnosis of chromosomal abnormalities both basic and
immunohistochemical study showed H.c. presence in all specimens.
In cases of gestational diabetes mellitus both basic H - E and
immunohistochemical study showed H.c. presence in 70% of cases.
In IUGR pregnancies,basic study was positive for H.c. presence in 62.5% of
cases, while immunohistochemical study using A1 Antichymotrypsine revealed H.c.
in all specimens (100%).
This study is in agreement with recent literature reports which support that
H.c. are fetal tissue macrophages, present in placental villi during pregnancy,
but with progressively reducing concentration.
Keywords:
Hofbauer cells, immunohistochemistry, pathologic gestations, placenta, macrophages
File:
File access is restricted.
document.pdf
3 MB
File access is restricted.
