Unit:
Κατεύθυνση Βιολογία Στόματος (Βασικές Οδοντιατρικές Επιστήμες)Βιβλιοθήκη Οδοντιατρικής
Author:
Ανδρίκουλα Θεοφανή
Supervisors info:
Κιτράκη Ευθυμία, Βασταρδή Ελένη, Ουλής Κωνσταντίνος
Original Title:
Ανίχνευση μοριακών αλλαγών σε ανθρώπινα οδοντικά πολφικά κύτταρα σε μοντέλα κυτταρικής γήρανσης
Summary:
This study examined the changes in the levels of proteins associated with
cellular stress and calcification of dentine, in two models of cellular
senescence of dental pulp cells. For this purpose we used primary cultures of
dental pulp cells derived from human impacted third molars. The induction of
replicative senescence was achieved upon 20 successive passages and the
confirmation of cellular aging was done by detecting the senescence associated
β-galactosidase (SA β-gal)-positive cells. The induction of stress-induced
premature senescence was performed by exposing the cells to 150 μM H2O2 for 2
hours. The sub-cytotoxic effect of H2O2 on cell viability was evaluated by
using the MTT method. Protein levels of thioredoxin (TRX1), ERdj5 and dentin
sialoprotein (DSP) were determined by Western blot at different passages (model
of replicative senescence) and at different time intervals following H2O2
treatment (model of stress-induced premature senescence) and were compared with
respective protein levels in young cells.
Our results showed that replicative senescence led to a general reduction in
the levels of all proteins studied. On the other hand, the stress-induced
premature senescence resulted in a time-dependent increase of TRX1 levels upon
exposure to H2O2.
In conclusion, this study provides some evidence that replicative senescence
and stress-induced premature senescence of dental pulp cells exhibit different
cellular responses at the protein level.
Keywords:
Dental pulp, Oxidative stress, Aging, Endoplasmic reticulum stress
