Unit:
Κατεύθυνση Ενδοδοντία (Κλινικές Ειδικεύσεις)Βιβλιοθήκη Οδοντιατρικής
Supervisors info:
Κα Ευθυμία Κιτράκη, Καθηγήτρια Βιολογίας της Οδοντιατρικής Σχολής του Πανεπιστημίου Αθηνών, Κα Μαρία Γεωργοπούλου, Αναπληρώτρια Καθηγήτρια Ενδοδοντίας της Οδοντιατρικής Σχολής του Πανεπιστημίου Αθηνών, Κος Μαρουάν Χαμπάζ, Αναπληρωτής Καθηγητής & Διευθυντής Εργαστηρίου Ενδοδοντίας της Οδοντιατρικής Σχολής του Πανεπιστημίου Αθηνών.
Original Title:
Αξιολόγηση της επίδρασης του βιοσυμπλέγματος ανθρώπινων οδοντικών πολφικών κυττάρων/ ικριώματος εξωκυττάριας μήτρας στην επούλωση κρανιακών οστικών ελλειμμάτων σε επίμυες
Summary:
The rat calvarial defect is an established model to evaluate craniofacial bone
regeneration using
cell-scaffold biocomplexes. Dental pulp harbors stem cells with significant
osteogenic properties.
Extracellular matrix (ECM)-like scaffolds simulate the environment that cells
observe in vivo.
In the present study, we evaluated the osteogenic effect of a biocomplex of
human dental pulp
cells and a hyaluronic-based hydrogel scaffold in calvarial defects of
immunocompetent rats.
Dental pulp cells at the 2nd passage were characterized by flow cytometry,
osteodifferentiated ex
vivo for 4 days and the whole population was encapsulated in the synthetic ECM
matrix. Cell
vitality was verified 24h upon encapsulation. 5 mm calvarial defects were
created in 15 male rats
and filled with the biocomplex, the scaffold alone, or nothing.
Histological evaluation at 8 weeks showed incomplete bone regeneration in all
groups. The
scaffold was not fully degraded and entrapped cells were detected in it.
Histomorphometry
showed statistically significant superior new bone formation in the
biocomplex-treated group and
similarly lower regeneration in the two other groups.
The present study provides evidence that the whole population of human dental
pulp cells
combined with an ECM-based scaffold can advance bone healing when transplanted
in
immunocompetent animals.
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