Κατεύθυνση Βιομηχανική Φαρμακευτική
Library of the School of Science

2014-04-04

2014

Λιάκος Σωτήριος

Μιχάλης Ράλλης Επίκ. Καθηγητής ΕΚΠΑ (επιβλέπων), Τεντολούρης Νικόλαος Αναπλ. Καθηγητής ΕΚΠΑ, Κλέτσας Δημήτριος Ερευνητής Α' ΕΚΕΦΕ "Δημόκριτος"

Αξιολόγηση Πρωτότυπων Σκευασμάτων στην Επούλωση του Δέρματος των Διαβητικών Μυών

Greek

Evaluation of Novel Normulations in Skin Wound Healing of Diabetic Mice

Diabetes mellitus is a group of metabolic diseases characterized by
hyperglycemia resulting from defects in insulin secretion, insulin action, or
both. Diabetes related with complications such as retinopathy, nephropathy,
neuropathy and early atherosclerosis. Among the complications isthe diabetic
foot which could affect the glycemic status and quality of life of diabetic
patients. Oxidative stress and free radical of oxygen and nitrogen have been
found to play an important role in the pathogenesis of diabetes and its
complications.
Wound healing is one of the major problems in the daily life and quality of
life of many people. There is concern in chronic wounds which do not heal
quickly and sometimes also lead to amputation or gagrene. The wounds of
diabetic patients are referred as chronic and / or impaired. Despite the
progress in the study of wound healing with modern techniques, little progress
has occurred in the discovery of new and effective formulations. The aim of
this work was to find new therapeutic agents for the treatment of diabetic
wound .
In this study, were tested about 30 different preparations applying on mice in
order to evaluate the wound healing ability. The main origin of these
formulations is from manuscripts of 17th-18th century AD and traditional
medicine and natural or chemical products. The 3 best ones were tested on skin
of diabetic mice .
The experiment was divided into two phases . In the first screening phase were
examined the wound healing activity of 30 different preparations in groups of
three normal mice each. In this study was caused skin trauma (1cm2in size) and
applied the formulations daily using gauze and transparent adhesive membrane.
The evaluation of the healing activity was done with clinical measurements
(weight, temperature and standardized visual assessment), pictures and
measurements of quality characteristics of the skin, such as transepidermal
water loss, hydration, elasticity, melanin and redness levels. In the second
phase the 3 best formulationswere selected and tested in diabetic mice compared
to normal and diabetic mice.The quality of wound healing correlated with non-
invasive methods. Additional measurements were made such as histological
evaluation of the skin, damage to the deoxyribonucleic acid by comet assay and
lipid peroxidation by TBARS in the skin and plasma.
The results of the study, showed that a formulation which contained extract of
Ceratothoa Oestroides gave the best results in wound closure. Furthermore it
was shown that both high glucose levels and age play an important role in wound
healing. Measurements in the biophysical properties of skin showed disfunction
of the skin barrier, reduced hydration and increased melanin and redness.
Several of the formulations are interesting for further studies.

Healing, Diabetes, Skin, Mice, Wound

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1-11

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https://pergamos.lib.uoa.gr/uoa/dl/object/1316270