Biochemical analysis and Molecular typing of Candida species in vaginal samples

Postgraduate Thesis uoadl:1316593 189 Read counter

ΠΜΣ Μικροβιακή Βιοτεχνολογία
Library of the School of Science
Deposit date:
Βαλασοπούλου Μαριάννα
Supervisors info:
Χατζηνικολάου Δημήτριος Επίκ. Καθηγητής
Original Title:
Βιοχημική ανάλυση και μοριακή τυποποίηση του είδους Candida σε κολπικά δείγματα.
Translated title:
Biochemical analysis and Molecular typing of Candida species in vaginal samples
Over the last 20 years there have been significant changes in the capital of
mycoses regarding on the frequency causes and pathogenesis. Although it is
estimated that the total number of known and unknown species of fungi exist on
our planet is about 1.5 million have been described about 300,000 species, of
which only 600 were found to be opportunistic pathogens for humans and less
than 100 belong to the usual pathogens (causing mycoses mucous membranes, skin,
soft tissue, nails, fur, and other diseases such as allergies or fatal invasive
infections). The species of the genus Candida is the most common fungal species
detected in vaginitis. There is a great lack of ability to identify
phylogenetically different species of the genus Candida as they have many
common features. Microscopy alone, or the implementation of other laboratory
methods, beyond the precise molecular characterization within the Candida
species complexes, such as the common pathogenic complex species C.
parapsilosis and C. glabrata, is inefficient and often inaccurate. This study
tested 134 vaginal samples. The 61 were positive having classical picture
vulvovaginitis and attended as outpatients for consideration at the General
Hospital of Athens "ELENA VENIZELOU". The isolation of Candida species from
clinical material was made by culturing the vaginal smear on sabouraud dextrose
agar. The characterization of the isolated strains of Candida was firstly by
biochemical tests. The samples were subjected to biochemical tests API ID32C
(BioMerieux) and the results showed that, four different Candida species were
vulvovaginitis causes in 61 patients tested. Further present in proportion 54%
C. albicans, 38%, C. parapsilosis 5%, C. Glabrata and 3% C. krusei. It was
followed by the formulation of the species Candida, which was isolated from 61
patients, analysis of allelic polymorphism of the length of minisatellite DNA
(MLP- Microsatellite Length Polymorphism), highly accurate technique for
determining the intraspecies variation of species of Candida. The results of
the standardization of the samples studied showed intraspecific differences of
the genus C. albicans and no intraspecific differences in the species C.
parapsilosis, C. krusei and C. glabrata.
Candida, Vulvovaginitis, Fungities, Diagnosis
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