Unit:
Τομέας ΦΑΡΜΑΚΟΓΝΩΣΙΑΣ ΚΑΙ ΧΗΜΕΙΑΣ ΦΥΣΙΚΩΝ ΠΡΟΪΟΝΤΩΝLibrary of the School of Science
Supervisors info:
Φωκιαλάκης Νικόλαος Επίκ. Καθηγητής
Original Title:
Διερεύνηση των συνθηκών καλλιέργειας του μυκητόφιλου μύκητα Mycogone rosea ως προς την παραγωγή δευτερογενών μεταβολιτών
Translated title:
Investigation on the culture conditions of the mycophilic fungus Mycogone rosea for the production of secondary metabolites
Summary:
Fungi are widely known for their ability to produce bioactive small molecules
otherwise known as secondary metabolites. Production of fungal secondary
metabolites is a special process that occurs only under specific environmental
conditions or at a specific stage in their life cycle.The ability of use
epigenetic modifying factors, in order to enhance the secondary metabolism of
fungi, is an application that has recently been studied. In continuation of our
studies for the discovery of novel secondary metabolites from the mycophilic
strain Mycogone rosea we investigated the effect of using inhibitors of DNA
methyltransferases (DNMT) and histone deacetylase (HDAC) and the effect of
using synthetic adsorption resin in the production of secondary metabolites in
liquid and solid cultures. Furthermore, we investigated the profile after
culture in fifteen different nutrients. The fungus was cultured in the presence
and absence of chemical epigenetic factorsand resin. The metabolic profile of
the extracts was studied by HPTLC, FCPC, MPLC, ESI-MS, GC-MS, HPLC, NMR methods
and low-pressure chromatography and found that the DNA methyltransferase
inhibitor and the use of adsorbent resin enhanced not only the overexpression
of specific molecules but also the production of new secondary metabolites.
Finally, accomplished biological control in which the fungi exhibited
antibacterial activity against the bacteria Escherichia coli and Micrococcus
luteus.
Keywords:
Mycogone rosea, Epigenetics, Antibacterial activity, Naphthoquinone, Sesquiterpenic amide
Number of index pages:
6-7
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