Κατεύθυνση Κλινική Χημεία
Library of the School of Science

2013-02-26

2013

Μπόνη Χρυσοθέμις

Ευρίκλεια Λιανίδου Καθηγ. ΕΚΠΑ (Επιβλέπουσα), Πίντζας Αλέξανδρος Ερευνητής Α΄ ΕΙΕ, Γεωργία Σωτηροπούλου Αναπλ. Καθηγ.Πανεπιστήμιο Πατρών

Επιγενετικές αλλοιώσεις στην καρκινική διείσδυση και μετάσταση μέσω των επιγενετικών παραγόντων ΕΖΗ2 και microRNA-21 σε κυτταρικά μοντέλα και κλινικά δείγματα του παχέος εντέρου

Greek

Epigenetic modifications in cancer invasion and metastasis through the epigenetic factors EZH2 and microRNA-21 in colon cell models and clinical samples

In the present Master of Science diploma, the biological and the clinical
importance of the epigenetic factors EZH2 and miR-21 has been investigated in
colon cancer. Our study was separated in two parts:In the first part, that was
focused at the cell biology level, it has been inferred the association of the
epigenetic factor EZH2, an histone methyltransferase, with the metastatic
properties of cancer cells.The expression of RHOA, ROCK1, RAC1, CDC42, was not
changed in colon cancer cell line, after the suppression of EZH2. The
investigation of expression levels of cofilin, the last effector of the RHO
GTPases family, revealed that its phosphorylation levels increased, after
silencing of EZH2 resulting in a reduction of the metastatic ability.In the
second part that was focused at the clinical level, the clinicalpathological
importance of the epigenetic factor miR-21 and its target gene, PDCD4, was
investigated in colon cancer tissues. Both genes, miR-21 and PDCD4 were over
and under expressed respectively, in tumor samples when compared to normal.When
The expression levels of both genes, were correlated with the presence of
metastasis or the presence of mutations in the MAPK pathway no association was
found.In conclusion, our data indicate that the epigenetic factor EZH2 is
involved in the metastatic ability of cancer cells via cofilin, whereas the
epigenetic factor miR-21 in combination with gene PDCD4 should be further
evaluated as potential tumor markers.

ΕΖΗ2, Cell invasion, Cofilin, RHO GTPase family, miR-21

Yes

1-11

Yes

123

155

File access is restricted.

https://pergamos.lib.uoa.gr/uoa/dl/object/1317324