Τομέας Βιοχημείας Μοριακής Βιολογίας
Library of the School of Science

2013-02-27

2013

Τσάκου Αναστασία

Βασιλακοπούλου Διδώ Αναπλ. Καθηγ. (Επιβλέπουσα), Σίδερης Διαμάντης Αναπλ. Καθηγ., Σκορίλας Ανδρέας Αναπλ. Καθηγ.

Η επίδραση της L-Dopa αποκαρβοξυλάσης στην απόπτωση

Greek

Study ypon the effect of L-Dopa decarboxylase in apoptosis

According to recent studies in our lab, L-Dopa decarboxylase (DDC, EC
4.1.1.26), is not only responsible for the decarboxylation of L-Dopa to
dopamine, but
may also be involved in apoptosis. This study investigated 1) characteristics
of the
involvement of L-Dopa decarboxylase in apoptosis, 2) the possible involvement of
alternative human DDC isophorms in apoptosis and 3) the interaction of the
enzyme
with molecules that participate in apoptotic mechanisms, in non-apoptotic and
apoptotic
conditions.
First of all, we studied the effect of human L-Dopa decarboxylase and its
isophorms in apoptosis, by stayrosporine (STS) – induced apoptosis in human cell
lines: HeLa, HEK293, SH-SY5Y, CHO/DDC-3 and CHO/Alt-DDC. Our results
underline the further involvement of L-Dopa decarboxylase in apoptosis, as well
as the
participation of its alternative isophorms in apoptotic mechanisms.
Moreover the interaction of DDC with molecules involved in apoptosis was
studied. Co-immunoprecipitation experiments had showed the interaction of L-Dopa
decarboxylase with annexin V and Bcl-2 protein. The compare of results in
nonapoptotic
and apoptotic conditions, indicated quantitative and qualitative differences in
the interaction, among HeLa, HEK293 and SH-SY5Y cell lines, confirming the
involvement of DDC in apoptotic mechanisms.

Apoptosis, L-Dopa decarboxylase, Annexin V, Bcl-2, Immunoprecipitation

No

0

Yes

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https://pergamos.lib.uoa.gr/uoa/dl/object/1317707