Summary:
The effect of tobacco smoke on health is a subject of major concern
particularly in the last decades, because of the increasing number of smokers
worldwide and of the knowledge of its harmful effects on human health.
Cigarette smoke over many pathological conditions and diseases with it is
connected, seems to be associated with many skin pathologies such as premature
ageing of skin, impaired wound healing, skin cancer, etc.
Cigarette smoke, according to studies made is phototoxic, and there are
conditions that the photo toxicity may be the mechanism by which cigarette
smoke causes premature skin ageing and enhances photo carcinogenicity.
Especially, UVA radiation is associated with oxidative damage associated with
photoageing. Studies have shown that exposure to cigarette smoke followed by
exposure to ultraviolet radiation synergistically increases the breaking of DNA
strands and lipid peroxidation in human white blood cells.
In our study, the toxic effect of tobacco smoke and of ultraviolet
radiation UVA and UVB is examined on cultured keratinocytes. Additional, it is
examined the protection afforded against irradiation (with UVA and UVB) on
keratinocytes culture, of innovative anti-inflammatory compounds with
antioxidant activity. Further, it is examined the possible protective effect of
two antioxidants, extracts of cranberry and mill wastewater. Keratinocytes were
obtained from newborn hairless mice type SKH-HR1 and SKH-HR2. The oxidative
stress was evaluated by fluorescene microscopy and fluorescence measurements.
The tobacco smoke and the ultraviolet radiation produce oxidative
stress, which is estimated by fluorescence microscopy and measurements. In the
presence of cigarette smoke, the loss of cells was very high. Regarding the
antioxidants used, the presence of mill effluents seemed to provide protection
to keratinocytes in the presence of oxidants studied. Finally, with regard to
anti-inflammatory compounds used, the compound AK89 appears to have the
greatest protective effect against irradiation.
Keywords:
CIGARETTE SMOKE, UV LIGHT, IN VITRO, KERATINOCYTES, OXIDATIVE STRESS
