Κατεύθυνση Κλινική Χημεία
Library of the School of Science

2014-11-05

2014

Μεγαδούκας Πέτρος

Λιανίδου Ευρύκλεια Καθηγήτρια

Λιπιδωμική ανάλυση φωσφολιπιδίων σε χρόνιες φλεγμονώδεις παθήσεις (πνευμονική ίνωση, άσθμα,ηπατίτιδα)

Greek

Lipidomic analysis of phospholipids in chronic inflammatory diseases (pulmonary fibrosis, asthma, hepatitis)

The lipidomic analysis, ‘’lipidomics’’, in biological samples and the
acquisition of a specific lipid profile, provides information concerning the
changes in the composition of several lipid species and reveals potential
biomarkers and therapeutic targets for various diseases, such as inflammatory
ones. In this research project, we studied the phospholipid profile in
pulmonary fibrosis, asthma, and hepatitis. This study constitutes the first
attempt, internationally, to correlate the level of the major subclasses of
lysophospholipids in the prognosis of these diseases. For this purpose, we
developed a method of LC coupled with mass spectrometer LTQ Orbitrap XL®
(HPLC-MS/MS) for the simultaneous analysis of all phospholipids (monoacyl and
diacyl), sphigolipids and certain glycerolipids (TG and DAG). We used animal
models of disease, as well as blood samples and BALF from patients. The results
of this specific study indicates that the metabolism of phospholipids
significantly had been affected in toxic hepatitis and pulmonary fibrosis, in
spite of mild asthma that did not appear to be significantly affected. The
biological significance of changes of phospholipids will be further examined to
enlighten whether the change in the concentration of lipids is only the result
of damage or otherwise it involves in the pathogenesis.

Lipids, Mass spectrometry (MS), Inflammatory diseases, Pulmonary fibrosis, Asthma

Yes

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https://pergamos.lib.uoa.gr/uoa/dl/object/1318213