Immunohistochemical study of the expression of Line-1 (ORF2p) and APOBEC3B proteins in the labial minor salivary glands of Sjögren's syndrome patients

Postgraduate Thesis uoadl:1325690 273 Read counter

Unit:
Κατεύθυνση Στοματολογία (Κλινικές Ειδικεύσεις)
Βιβλιοθήκη Οδοντιατρικής
Deposit date:
2016-12-05
Year:
2016
Author:
Kalogirou Eleni-Marina
Supervisors info:
Ευαγγελία Πιπέρη, Επίκουρη Καθηγήτρια, Τμήμα Οδοντιατρικής , ΕΚΠΑ
Σκλαβούνου Αλεξάνδρα, Καθηγήτρια, Διευθύντρια Εργαστηρίου Στοματολογίας, Τμήμα Οδοντιατρικής , ΕΚΠΑ
Τόσιος Κωνσταντίνος, Επίκουρος Καθηγητής, Τμήμα Οδοντιατρικής , ΕΚΠΑ
Original Title:
Immunohistochemical study of the expression of Line-1 (ORF2p) and APOBEC3B proteins in the labial minor salivary glands of Sjögren's syndrome patients
Languages:
English
Translated title:
Ανοσοϊστοχημική διερεύνηση της έκφρασης των μορίων Line-1(ORF2) και APOBEC3B σε ελάσσονες σιαλογόνους αδένες κάτω χείλους ασθενών με σύνδρομο Sjögren
Summary:
Objective: The aim of this study was to investigate the immunohistochemical expression of L1NE-1 ORF2 and APOBEC3B in the minor salivary glands (MSGs) of Sjögren’s syndrome (SS) patients.
Methods: L1ORF2p and APOBEC3B expression was assessed by immunohistochemistry in MSG tissues from 20 SS patients, classified according to Tarpley’s histological criteria, 5 chronic sialadenitis (CS) controls and 5 normal MSG (NMSG) controls.
Results: L1ORF2p was expressed in 17/20 SS patients and all controls, while APOBEC3B expression was observed in 15/20 SS, 5/5 CS and 3/5 NMSG cases. Both antibodies stained the cytoplasm of the ductal epithelial cells, while negative staining was observed in the periductal lymphocytic infiltration in SS patients and the chronic inflammation in the controls. A statistically significant difference was found between the L1ORF2p immunostaining in Tarpley IV SS patients and the controls (p=0.038), as well as between the APOBEC3B expression in Tarpley I SS patients and the controls (p=0.035).
Conclusions: The expression of L1 ORF2p in MSGs promotes the role of L1 element as intrinsic antigen in SS. The L1ORF2p and APOBEC3B co-expression in the ductal epithelium of SS patients possibly reflects the role of APOBEC3 enzymes as an inhibitory mechanism against the inappropriate endogenous expression of L1.
Main subject category:
Health Sciences
Keywords:
Sjögren’s syndrome, minor salivary glands, LINE-1 ORF2p, APOBEC3B
Index:
No
Number of index pages:
0
Contains images:
Yes
Number of references:
450
Number of pages:
224
Kalogirou_Thesis 2016.pdf (12 MB) Open in new window