Unit:
ΠΜΣ Αναπαραγωγική-Αναγεννητική ΙατρικήLibrary of the School of Health Sciences
Author:
Asimakopoulos Georgios
Supervisors info:
Λουτράδης Δ., Καθηγητής, Ιατρική, ΕΚΠΑ
Δρακάκης Π., Αναπληρωτής Καθηγητής, Ιατρική, ΕΚΠΑ
Στεφανίδης Κ., Αναπληρωτής Καθηγητής, Ιατρική, ΕΚΠΑ
Original Title:
Έκφραση του γονιδίου DAZL σε τροφοβλαστικά κύτταρα μετά από CVS
Translated title:
DAZL expression in trophoblastic cells derived by Chorionic Villus Sampling (CVS)
Summary:
The DAZL gene is a germ cell marker and it is expressed in embryonic stem cells. In recent years, the scientific community is looking for the importance of its expression in various embryonic and extra-embryonic tissues. The purpose of this study was to search the expression of DAZL gene and, in particular, the finding of gene expression or not in trophoblastic cells of the placenta. These cells were obtained by chorionic villus sampling (CVS), in the context of prenatal diagnostic testing at the end of the first trimester of pregnancy. The DAZL gene expression in trophoblastic cells was measured with the Real - Time PCR technique and demonstrated minimal gene expression, different from zero, which cannot be considered negligible. The comparison with other relevant studies revealed agreement of the results. Therefore, the finding of DAZL gene expression in trophoblastic cells seems to be related to the potential capacity of these cells to differentiate into germ cells under appropriate conditions, opening new paths in the fields of research and regenerative medicine.
Main subject category:
Health Sciences
Keywords:
DAZL, Trophoblastic cells, Placenta, Chorionic villus sampling (CVS), Germ cells
Number of references:
258
