Supervisors info:
Σκαλτσά Ελένη, Καθηγήτρια,Τομέας Φαρμακογνωσίας και Χημείας Φυσικών Προϊόντων, Τμήμα Φαρμακευτικής, Εθνικό Καποδιστριακό Πανεπιστήμιο Αθηνών
Summary:
In this thesis, chemical analyses of non volatile constituents derived from wild Crithmum maritimum L. (Apiaceae) and of the essential oil of cultivated populations were carried out. The plant material was selected from maritime cliffs in a small coastal town, Parga (W. Greece-Ionian sea) and the aerial parts have been extracted with cyclohexane, dichloromethane, methanol, methanol: water 5:1, successively. The phytochemical analyses, performed by means of analytical techniques and NMR spectroscopy, allowed us to isolate and identify ten compounds. From the cyclohexane extract three compounds were isolated belonging to different phytochemical groups namely falcarindiol (polyacetylene), O-geranylvanillin (monoterpene) and octadecanal (fatty aldehyde). From the methanol extract seven compounds were isolated: chlorogenic, caffeic and 3, 4-O-dicaffeoylquinic acids and the flavonoids rutin, quercetin-3-O--D-robinobioside, hyperoside and isoquercitrin. Furthermore, we investigated the chemical profiles of C. maritimum decoctions and infusions from wild populations from Greece and Italy. Finally, the essential oils (EOs) of the aerial parts obtained from cultivated populations of two consecutive years (2016; 2017; Larissa-Central Greece) were analyzed by GC-MS. The main compounds of the EO derived from the cultivar of 2016 were sabinene (17.6%), γ-terpinene (17.5%), p-cymene (16.7%), β-phellandrene (15.5%), thymol methyl ether (9.3%), terpinen-4-ol (4.8%), and dillapiole (2.0%), while in the sample of 2017 were β-phellandrene (30.9%), γ-terpinene (19.6%), sabinene (15.8%), thymol methyl ether (7.6%), terpinen-4-ol (4.8%), and dillapiole (0.2%). Finally, a comparison was made between the two samples, as well as with the literature data.
Keywords:
Crithmum maritimum, Apiaceae, Essential oil, Flavonoids, Phenolic acids, Polyacetylenes,monoterpenes
