Unit:
Κατεύθυνση Αναλυτική ΧημείαLibrary of the School of Science
Supervisors info:
Νικόλαος Θωμαΐδης, Καθηγητής, τμ. Χημείας, ΕΚΠΑ
Original Title:
Ανάπτυξη και επικύρωση μεθόδου προσδιορισμού αναδυόμενων ρύπων και ρύπων προτεραιότητας σε θαλάσσιους οργανισμούς με GC–MS/MS
Translated title:
Development and Validation of a determination method for Emerging contaminants and Priority Organic Pollutants in aquatic organisms by Gas Chromatography tandem Mass Spectrometry (GC-MS/MS) technique
Summary:
The determination of dangerous priority pollutants and persistent organic pollutants (POPs) in aquatic biota can provide with plenty information on the ability of those compounds to climb higher in the food chain while it is also a criterion for degradation of the aquifers.
Due to the effective intervention of environmental authorities, the past few decades, with the establishment of Framwork Directives for the protection of water quality and initiation of monitoring programs, the use of priority substances is in many cases restricted or eliminated. However, it is well known that such compounds are resilient, thus persist in air, aquatic and solid phase resulting in a tendency to bioaccumulate in living organisms and still be ubiquitous in ecosystems.
In the present thesis, a method for the determination of organochlorine pesticides (OCPs) and polycyclic aromatic hydrocarbons (PAHs) in aquatic biota samples is being developed and validated. This method was applied to 19 real specimens, from various sampling sites of Black Sea. The analysis of the aforementioned compounds was performed using gas chromatography tandem triple quadrupole mass spectrometry (GC-EI-QQQ). Quality assurance and quantitation was achieved with the use of Quality Control samples (QC) and Internal Standards (IS) for each analyte. Accuracy, Precision, Matrix Effect and Limits of Detection and Quantitation were used as performance characteristics.
The exposure extent of each biota sample varied not only depending on the lipid content of the sample but the sampling site as well. Some of the most characteristic analytes present in all the biota samples, were: Pyrene from PAHs group, with concentrations ranging from 0.19 to 16 μg/kg and DDT group (including DDD and DDE compounds) with concentrations ranging from 1.3 to 18 μg/kg.
Main subject category:
Science
Keywords:
priority pollutants, OCPs, PAHs, aquatic biota, GC–EI–QQQ
