Evaluation of red wine polyphenols extract on the prevention of osteoporosis in estrogen deficient ovariectomized rats.

Doctoral Dissertation uoadl:2874903 417 Read counter

Faculty of Medicine
Library of the School of Health Sciences
Deposit date:
Pasali Christina
Dissertation committee:
Ισμήνη Νίκη Δοντά, Καθηγήτρια, Ιατρική Σχολή, ΕΚΠΑ
Αλέξιος - Λέανδρος Σκαλτσούνης, Καθηγητής, Τμήμα Φαρμακευτικής, ΕΚΠΑ
Νικόλαος Παπαϊωάννου, Αναπληρωτής Καθηγητής, Ιατρική Σχολή, ΕΚΠΑ
Σοφία Μητάκου, Καθηγήτρια, Τμήμα Φαρμακευτικής, ΕΚΠΑ
Ευανθία Κασσή, Αναπληρώτρια Καθηγήτρια, Ιατρική Σχολή, ΕΚΠΑ
Νεκτάριος Αληγιάννης, Αναπληρωτής Καθηγητής, Τμήμα Φαρμακευτικής, ΕΚΠΑ
Ιωάννης Τριανταφυλλόπουλος, Επίκουρος Καθηγητής, Ιατρική Σχολή, ΕΚΠΑ
Original Title:
Μελέτη της δράσης των φαινολικών ενώσεων του κρασιού στην πρόληψη της οστεοπόρωσης στο πειραματικό πρότυπο του θήλεος ωοθηκεκτομημένου επίμυος.
Translated title:
Evaluation of red wine polyphenols extract on the prevention of osteoporosis in estrogen deficient ovariectomized rats.
Τhe aim of this study was to examine the effect of Red Wine Polyphenols (RWPs) extract on bone mineral density (BMD) in the ovariectomized (OVX) rat model. Eleven of the 50 rats consisted the sham-operated non-ovariectomized control group (CTRL, n = 11). Thirty-nine rats underwent bilateral ovariectomy by the ventral approach; of these, 13 rats consisted the ovariectomized group without treatment (OVX, n  = 13), 11 rats were treated with 50 mg/kg body weight per day RWPs extract (RWPs Low, n = 11) and 15 rats were treated with 150 mg/kg RWPs extract (RWPs High, n = 15). Βody and uterine weight, BMD of the tibia at baseline, 3 and 6 months post-OVX, and 3-pointing bending of the femur, were examined. Statistical comparison of the proximal tibia BMD within groups during the study period showed a significant reduction in the OVX vs treated rats (RWPs Low and RWPs High) both from baseline to 3 and 6 months and from 3 to 6 months (Ρ < 0.05). For the proximal tibial metaphysis, comparison of BMD percentage changes from baseline to 3 months and to 6 months, revealed highly statistical differences between OVX and RWPs groups (OVX vs RWPs Low, P = 0.000 and P = 0.000 and OVX vs RWPs High, P = 0.000 and 0.000, respectively). OVX induced a significant reduction of biomechanical parameters as expected; energy absorption and significantly increased in the OVX group treated with RWPs at 50 mg/kg and 150 mg/kg, compared with the nontreated OVX rats (P = 0.007 and P = 0.033, respectively). Fracture stress was higher in the RWPs treated groups compared to the OVX group. Statistically significant results were obtained between RWPs Low and OVX (P = 0.016). RWPs High fracture stress measurements were higher than the OVX group, and similar to the CTRL group, however, no statistically significant findings were found. Dietary intake of RWPs for 6 months significantly prevented trabecular bone loss and improved bone strength in estrogen-deficient ovariectomized rats.
Main subject category:
Health Sciences
Osteoporosis, Rats, Polyphenols, Bone mineral density, Three point bending
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