Unit:
Κατεύθυνση Ενδοδοντία (Κλινικές Ειδικεύσεις)Βιβλιοθήκη Οδοντιατρικής
Author:
Papadopoulou Charalambia
Supervisors info:
Μαρία Γεωργοπούλου, Καθηγήτρια,Τμήμα Οδοντιατρικής, Σχολή Επιστημών Υγείας, ΕΚΠΑ
Παναγιώτης Πανόπουλος,Καθηγητής, Τμήμα Οδοντιατρικής, Σχολή Επιστημών Υγείας, ΕΚΠΑ
Ιωάννης Καρούσης,Αναπληρωτής Καθηγητής, Τμήμα Οδοντιατρικής, Σχολή Επιστημών Υγείας, ΕΚΠΑ
Original Title:
In vitro αξιολόγηση της βιοσυμβατότητας και κυτταροτοξικότητας ενός νέου βιοκεραμικού υλικού για ενδοδοντική χρήση
Translated title:
In vitro evaluation of the bioactivity and cytotoxicity of a new bioceramic material for endodontic use
Summary:
In present study the bioavailability and the cytotoxicity of Total Fill was tested in comparison with two well-known and well documented similar commercial bioceramic materials, ΜΤΑ and Biodentine.
To test bioavailability cell cultures of the above mentioned materials were performed using premature human osteoblasts of line MG63, which were in the 4th stage of re-cultivation, in conditions according with ISO 10993-5:2009(E). As control group received the cultivation of the MG63 cells in nutritious medium. All cell cultures made for 24h, 48h and 72h respectively. After the of cultivation time the number of proliferated cells was counted in the optical microscope, after dispersion in a Neubauer plaque, recording the cells of indicative quatrains and making the induction in the number of cells for all the plaque surface.
For cytotoxicity test the biomaterials were submitted in MTT assay and FDA and PI
colouring processes. Also a test of the ability for bone differentiation of osteoblasts cultivated on the tested biomaterials was performed, using one of the primary biochemical indexes of bone differentiation. At the end a qualitative determination of the cellular viability was made, using scanning electron microscopy.
Conclusively within the limitations of this in vitro study the new bioceramic material Total
Fill bioceramic material displayed excellent bioampatibility. In particular there was a
statistical significant increase (P=.002) of the cell number of Total Fill, compared to both
MTA and Biodentine, from the 24h to the 72h. However no significant differences of the cell population have been at the 48h and 72h respectively.
From the observation of SEM micrographs, it is evident that in the Total Fill bioceramic
material the MG63 cells maintain their typical morphology. Additionally, there are no signs of stressed cells during their effort to populate the material, in contrast to the others examined materials.
Main subject category:
Health Sciences
Keywords:
Biocompatibility, Cytotoxicity, Bioceramic, Total Fill
Number of references:
101
