Unit:
Κατεύθυνση Χημική Ανάλυση-Έλεγχος ΠοιότηταςLibrary of the School of Science
Supervisors info:
Βικτωρία Σαμανίδου (επιβλέπουσα), Καθηγήτρια, Τμήματος Χημείας, Α.Π.Θ.
Παπαδογιάννης Ιωάννης, Καθηγητής, Τμήματος Χημείας, Α.Π.Θ.
Θεοδωρίδης Γεώργιος, Καθηγητής, Τμήματος Χημείας, Α.Π.Θ.
Original Title:
Ανάπτυξη μεθόδου εκχύλισης προσρόφησης σε υφασμάτινο μέσο (FPSE) για τον προσδιορισμό πέντε αντικαταθλιπτικών φαρμάκων σε ορό ανθρώπινου αίματος με υγρή χρωματογραφία υψηλής πίεσης και ανιχνευτή παράταξης φωτοδιόδων
Translated title:
Development of fabric phase sorptive extraction (FPSE) method for the determination of five antidepressant drugs in human blood serum prior to high performance liquid chromatography with photodiode array detection
Summary:
In recent years, the research about synthesis of new antidepressant drugs and their dramatic increased consumption worldwide lead to the development of novel sample preparation techniques and analytical methods for the determination of these pharmaceuticals in biological samples.
In the current study, the new fabric phase sorptive extraction (FPSE) technique was applied for the first time in order to achieve the simultaneous extraction of five common antidepressant drugs (venlafaxine, paroxetine, fluoxetine, amitriptyline and clomipramine) from human blood serum. The examined compounds separation was carried out by a LiChrospher®100 RP-8e column (5 μm, 250 × 4 mm), using a mobile phase of ammonium acetate solution (0.05 mol/L) and acetonitrile in a 15 minutes gradient elution program. Detection was achieved by a photodiode array detector (PDA), monitoring at 235 nm. Elimination of protein precipitation step and minimized solvent consumption led to a sample preparation workflow compliant with the principles of green analytical chemistry. Among the different FPSE media examined, sol-gel polycaprolactone-dimethylsiloxane-polycaprolactone (sol-gel PCL-PDMS-PCL) coated polyester substrate presented optimum extraction efficiency and was found to be reusable for at least 30 times. Extraction and elution of the compounds was performed under stirring condition in 15 and 5 min, respectively, while a acetonitrile-methanol (50:50 v/v) mixture was used as the elution solvent. Recoveries ranged between 9.4% and 88.1% for all compounds, and relative standard deviations for intra-day repeatability and inter-day precision were ≤9.2% and ≤11.3%, respectively. The examined compounds in spiked blood serum samples were found to be stable for the conditions applied. Youden & Steiner approach was applied to study the method ruggedness.
Main subject category:
Science
Keywords:
Fabric phase sorptive extraction (FPSE), Blood serum, Antidepressant drugs, HPLC-PDA, Microextraction
