Unit:
Department of ChemistryLibrary of the School of Science
Author:
Kanioura Anastasia
Dissertation committee:
Χατζηχρηστίδη Μαργαρίτα, Επίκουρος Καθηγήτρια. Τμήμα Χημείας, ΕΚΠΑ
Κακαμπάκος Σωτήριος, Ερευνητής Α, Ε.Κ.Ε.Φ.Ε ΔΗΜΟΚΡΙΤΟΣ
Γογγολίδης Ευάγγελος, Ερευνητής Α, Ε.Κ.Ε.Φ.Ε ΔΗΜΟΚΡΙΤΟΣ
Πέτρου Παναγιώτα, Ερευνήτρια Α. Ε.Κ.Ε.Φ.Ε ΔΗΜΟΚΡΙΤΟΣ
Οικονόμου Αναστάσιος, Καθηγητής, Τμήμα Χημείας, ΕΚΠΑ
Εμμανουηλίδου Ευαγγελία, Επίκουρος Καθηγήτρια, Τμήμα Χημείας, ΕΚΠΑ
Κόκκινος Χρήστος, Επίκουρος Καθηγητής, Τμήμα Χημείας, ΕΚΠΑ
Original Title:
Ανάπτυξη βιοαναλυτικού μικροσυστήματος με νανοδομημένες επιφάνειες για τον εμπλουτισμό και την ανίχνευση καρκινικών κυττάρων
Translated title:
Development of a bioanalytic microsystem using nanostructured surfaces for the enrichment and the detection of cancer cells
Summary:
According to Centers for Disease and Control Prevention, cancer is the second cause of death worldwide and 90% of morbidity is due to metastasis. For this reason, the detection of the initiate tumor, as well as monitoring of metastasis is of great importance. Towards this direction, a lot of methods targeting the detection of cancer cells from blood or solid tumors have been employed. The aim of the current PhD thesis, was the development of a microsystem with embedded micro/nanostructured surfaces for the enrichment of cancer cells when in mixtures with normal ones. The first step of this study, was to investigate the effect of micro/nanostructuring on adhesion, proliferation and morphology of normal skin and lung fibroblasts and cancer cells (skin, prostate, lung). Thus, poly(methyl methacrylate) (PMMA) substrates (films and plates) were etched with oxygen plasma, under different conditions. It was found that, on the surfaces with mean height of approximately 1 μm (for the films) and 5.5 μm (for the plates), all cancer cell lines tested, adhered and proliferated unobstractly, while the proliferation of normal fibroblasts was hindered. Based on the different behavior of normal and cancer cells, co-culture experiments were performed on the above surfaces and an enrichment of cancer cells towards the normal ones, of up to 80 times was achieved, using the micro/nanostructured surfaces. Taking into account, the enrichment of cancer cells obtained on plain culture, co-culture experiments were also performed on a microfluidic channel, which embedded the micro/nanostructured surfaces. The results were very promising, concerning the enrichment of cancer cells when in mixtures with normal ones, under an optimum flow rate.
Main subject category:
Science
Keywords:
micro/nanostructuring, oxygen plasma, cancer cell enrichment, fibroblasts
Number of references:
198
