Faculty of Medicine
Library of the School of Health Sciences

2020-01-03

2019

Nosi Effrosyni

Αναστασία Κωνσταντινίδου, Καθηγήτρια, Τμήμα Ιατρικής, ΕΚΠΑ, Eπιβλέπουσα
Ανδρέας X. Λάζαρης, Καθηγητής, Τμήμα Ιατρικής, ΕΚΠΑ
Γεωργία Θωμοπούλου, Αναπληρώτρια Καθηγήτρια, Τμήμα Ιατρικής. ΕΚΠΑ
Ουρανία Τσιτσιλώνη, Καθηγήτρια, Τμήμα Βιολογίας, ΕΚΠΑ
Αφροδίτη Νόννη, Αναπληρώτρια Καθηγήτρια, Τμήμα Ιατρικής, ΕΚΠΑ
Νικόλαος Καβαντζάς, Καθηγητής, Τμήμα Ιατρικής, ΕΚΠΑ
Χρήστος Αλαμανής, Αναπληρωτής Καθηγητής, Τμήμα Ιατρικής, ΕΚΠΑ

Mελέτη δυνατοτήτων βελτίωσης της απόδοσης των σπερματεγχύσεων με παρεμβάσεις σε επιμέρους αιτιολογικούς παράγοντες που συντελούν στην πτώση της ποιότητας του σπέρματος

Greek

Study of the potential of improving the sperm quality by intervening in causative factors that contribute to the decline in sperm quality

Semen hyperviscosity impairs sperm motility and can lead to male infertility. This prospective study aimed at assessing the ability of exogenous DNase in improving sperm quality, taking into consideration that DNase has been found in the seminal plasma of several species and that neutrophils release chromatin in order to trap bacteria.
A total of seventy-seven semen samples with high seminal viscosity (HSV) as the study group and sixty-two semen samples with normal seminal viscosity (NSV) as the control group were compared in this analysis. These semen samples were divided into three groups of receiving treatment a) with DNase I at 37oC for 15 min, b) by Density Gradient Centrifugation and c) with a combination of the above two methods.
Following a fifteen-minute treatment of hyperviscous semen, the motility of spermatozoa in 83% of semen samples, increased to a statistically significant degree. On the contrary, DNase treatment of semen with normal viscosity had no such effects. The above treatment was also accompanied by a significant increase in the percentage of normal spermatozoa, resulting in a major decrease of the teratozoospermia index. Comparison between semen samples that underwent density gradient centrifugation following DNase I treatment, to those collected after density gradient treatment alone, showed that in the first case the results were more spectacular. The evaluation of each preparation in terms of yield (% total progressively motile sperm count after treatment in relation to the initial total sperm count) revealed that the combined approach resulted in 29.8% vs.18.5% with density treatment alone (p= 0.0121). DNase-I treatment results in an improvement of sperm motility and morphology and could be beneficial to men with hyperviscous semen in assisted reproduction protocols.

Health Sciences

Assisted reproductive technology (ART), Density gradient centrifugation, DNase I, Neutrophil extracellular traps (NETs), Semen hyperviscosity (SHV)

No

0

Yes

177

147

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https://pergamos.lib.uoa.gr/uoa/dl/object/2892992