Κατεύθυνση Φαρμακευτική Ανάλυση - Έλεγχος ποιότητας
Library of the School of Science

2020-02-12

2020

Koutserinaki Eleftheria

Λουκάς Ιωάννης, Αναπληρωτής Καθηγητής Φαρμακευτικής Χημείας, Φαρμακευτική, Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών

Ανάλυση Ιών HPV με χρήση Vision Array Chips και Real Time PCR

Greek

HPV analysis using Vision Array Chips and Real Time PCR

Human Papillomaviruses (HPV) are small sized viruses without an outer shell. Their genome consists of about 8,000 bases with a circular double stranded DNA. It has been identified more than 130 different types of HPV that contaminate the basic layer of skin’s papilloma and mucosa’s genital system. They cause many malfunctions such as a common papilloma or even cancer. It has been deduced that a group of HPVs can be the main reason of cervical papilloma because the DNA of this virus has been detected in the 99% of tumors. The types of the virus that contaminate the papilloma cells of genital system are split into three categories based on the risk that they cause: High-Risk, Probably High-Risk and Low-Risk. On the one hand there are High Risk HPV types which cause cancer of cervical papilloma. The main types of High-Risk HPV are 16, 18, 31, 33 and 45. On the other hand, Low-Risk types cause warts and their main types are 6 and 11. HPV are sexually transmitted. About 2/3 of people that have sexual intercourse with a contaminated person have possibly been contaminated by HPV, too. The contamination of High-Risk type of HPV can also cause stomach-pharynx cancer. HPV 16, 18, 45 and 31, are the most common types that cause cervical cancer. Test PAP is a technique that detects these types of HPV and as a result it has reduced the percentage of HPV incidents. However, the percentage of HPV contaminated people is still high. The scope of this thesis is to detect HPV by two different methods: Vision Array Chips and Real Time PCR. These two methods have been applied to 20 different patients in order to compare and contrast their results. Lastly, we conclude our results and then we choose the appropriate method for each patient.

Science

HPV, PCR, Real Time PCR, Vision Array Chips

Yes

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Yes

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https://pergamos.lib.uoa.gr/uoa/dl/object/2896873