Unit:
Κατεύθυνση Χημική Ανάλυση-Έλεγχος ΠοιότηταςLibrary of the School of Science
Supervisors info:
ΕΠΙΒΛΕΠΩΝ ΚΑΘΗΓΗΤΗΣ: ΜΙΧΑΗΛ ΚΟΥΠΠΑΡΗΣ, Καθηγητής ΕΚΠΑ
Original Title:
ΕΠΙΚΥΡΩΣΗ ΜΕΘΟΔΟΥ HPLC ΓΙΑ ΤΟΝ ΤΑΥΤΟΧΡΟΝΟ ΠΡΟΣΔΙΟΡΙΣΜΟ ΑΔΡΕΝΑΛΙΝΗΣ – ΛΙΔΟΚΑÏΝΗΣ ΣΕ ΕΝΕΣΙΜΑ ΦΑΡΜΑΚΕΥΤΙΚΑ ΣΚΕΥΑΣΜΑΤΑ
Translated title:
VALIDATION OF HPLC METHOD FOR SIMULTANEOUS DETERMINATION OF ADRENALINE – LIDOCAINE IN INJECTABLE MEDICINAL PRODUCTS
Summary:
In this Master Thesis, a method of simultaneous quantification of two active substances - adrenaline and lidocaine - has been developed, in medicinal products. Many methods have been developed for the determination of adrenaline and lidocaine in different medicinal products. The evolution in pharmaceutical technology and the ever-increasing need for new formulations containing a combination of active substances to achieve the combination of their properties creates new analytical challenges.
The technique used is the high performance liquid chromatography (HPLC) with a UV-Vis detector and a PDA detector. The wavelength was selected based on the maximum absorption of the two compounds, which was at 205 nm.
Initially standard solutions of the active substances were prepared and analyzed, and then the method was applied to a medicinal product. For the validation of the method selectivity, linearity, sensitivity, precision and stability of solutions were evaluated.
The main objective of the work was to develop a simple, fast, repeatable and economical method for the separation and quantification of two active substances.
Main subject category:
Science
Keywords:
Development, Validation, Adrenaline, Lidocaine, High Performance Liquid Chromatography
