Unit:
Faculty of MedicineLibrary of the School of Health Sciences
Author:
Pantazis Theodoros-Leonidas
Dissertation committee:
Μισιακός Ευάγγελος, Καθηγητής , Ιατρική Σχολή, ΕΚΠΑ
Γιωτάκης Ιωάννης, Ομότιμος Καθηγητής, Ιατρική Σχολή, ΕΚΠΑ
Κωνσταντουλάκης Μανούσος, Καθηγητής, Ιατρική Σχολή, ΕΚΠΑ
Σέγγας Ιωάννης, Καθηγητής, Ιατρική Σχολή, ΕΚΠΑ
Μαραγκουδάκης Παύλος, Καθηγητής, Ιατρική Σχολή, ΕΚΠΑ
Ζωγράφος Γεώργιος, Καθηγητής, Ιατρική Σχολή, ΕΚΠΑ
Κυροδήμος Ευθύμιος, Επίκουρος Καθηγητής , Ιατρική Σχολή, ΕΚΠΑ
Original Title:
Μελέτη καρκινικών δεικτών στον καρκίνο του λάρυγγα
Translated title:
Study of biomarkers in laryngeal cancer
Summary:
Purpose:Tumor recurrence and distant metastasis are very common in laryngeal squamous cell carcinoma(LSCC). in this study we examined the potential prognostic value of microRNA-20b-5p
(miR-205-5p), a coomponent of the tumor related miR-106a/363 cluster.
Materials and methods: Total RNA was purified from 105 tissue specimens rexected from patients having undergone surgical treatment for primary LSCC. After in vitro polyadenylation and reverse transcription, a sensitive real time quantitative polymerase chain reaction (qPCR) methodology was applied for the relative quantification of miR-20b-5p levels. Then we proceeded with biostatistical analysis, seeking to assess the prognostic value of miR-20b-5p expression in LSCC.
Results: miR-20b-5p positivity constitues a predictor of inferior DFS and OS in LSCC(P<0.001 and P=0.002 respectively). The significant prognostic value of miR-20b-5p expression status seems to be independent of tumor size, histological grade, and TNM stage, as revealed by the multivariate bootstrap Cox regression analysis. Kaplan-Meier survival analysis showed also that miR-20b-5p expression status can stratify LSCC patients with non-infiltrated regional lymp nodes (N0) into two subgroups with distinct prognosis(P=0.004 and P=0.004, respectively).
Conclusions: The miR-20b-5p expression status is a promising molecular tissue biomarker in LSCC, with an independent prognostic value, and thus merits further validadtion in larger cohorts of patients.
Main subject category:
Health Sciences
Keywords:
Head and neck cancer, Micro RNA
File:
File access is restricted only to the intranet of UoA.
Diatribi+TH-L+PantazisPDF.pdf
5 MB
File access is restricted only to the intranet of UoA.
