Unit:
Κατεύθυνση Ρευματολογία - Μυοσκελετική ΥγείαLibrary of the School of Health Sciences
Supervisors info:
Δημήτριος Βασιλόπουλος, Καθηγητής, Ιατρική Σχολή, ΕΚΠΑ
Δημήτριος Μπούμπας, Καθηγητής, Ιατρική Σχολή, ΕΚΠΑ
Κλειώ Μαυραγάνη, Αναπληρώτρια Καθηγήτρια, Ιατρική Σχολή, ΕΚΠΑ
Original Title:
Μελέτη του δικτύου αναγνώρισης και επιδιόρθωσης της βλάβης του DNA σε ανθρώπινα λεμφομονοπύρηνα κύτταρα
Translated title:
The DNA Damage Response and Repair Network in human peripheral blood mononuclear cells
Summary:
The DNA Damage Response and Repair Network in human peripheral blood mononuclear cells
Pappa M.
A’ Propaedeutic and Internal Medicine Department, Medical School, National and Kapodistrian University of Athens
Introduction: Recent studies highlight that defects in DNA Damage Response and Repair Network are present in patients with systemic autoimmune diseases.
Aim: Our purpose is to study DDR/R network in patients with Rheumatoid Arthritis (RA) and Systemic Lupus Erythematosus (SLE) and to test the hypothesis that these patients show defects in this network.
Methods: Peripheral Blood Mononuclear Cells (PBMCs) were isolated from 20 patients with RA, 20 with SLE and 50 apparently healthy controls (HC). Clinical and laboratory data were obtained from each participant after informed consent. Endogenous DNA damage [Single Strand (SSBs) and Double Strand (DSBs) DNA breaks] was measured by alkaline comet assay.
Results: Increased levels of endogenous DNA damage were present in patients compared to healthy controls [Olive Tail Moment arbitary units: HC: 4.5±2.3 (1.5-10.7), RA: 11.8±6.9 (5.1-35.5), SLE: 8.2±4.5 (2.4-23.0), p<0.05]. Rheumatoid factor (RF) is related with OTM in patients with RA; however, no statistically significant correlations were observred in SLE subgroup.
Conclusions: Patients with systemic autoimmune diseases accumulate endogenous DNA damage, that is related with clinical and laboratory characteristics of these patinets.
Main subject category:
Health Sciences
Keywords:
Systemic autoimmunity, DNA damage, DNA repair
