Development and validation of a new FPSE-HPLC method for the quantification of several parabens in human plasma

Postgraduate Thesis uoadl:2928836 36 Read counter

Κατεύθυνση Φαρμακευτική Ανάλυση-Έλεγχος Ποιότητας
Library of the School of Science
Deposit date:
Parla Anthi
Supervisors info:
Ειρήνη Παντερή, Καθηγήτρια, Τμήμα Φαρμακευτικής, Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών
Original Title:
Ανάπτυξη και αξιολόγηση μεθόδου εκχύλισης προσρόφησης σε υφασμάτινο μέσο και υγροχρωματογραφιας υψηλής απόδοσης (FPSE-HPLC) για τον ποσοτικό προσδιορισμό parabens σε ανθρώπινο πλάσμα
Translated title:
Development and validation of a new FPSE-HPLC method for the quantification of several parabens in human plasma
Parabens are widely used to prolong the shelf life of foods, daily care products, and pharmaceutical preparations. These compounds are preservatives that exhibit a broad range of antimicrobial activity and chemical stability without imparting any taste or odor. The combination of these properties makes it relatively difficult to
find a preservative, which will be a satisfactory replacement for parabens. Several studies revealed that they are absorbed through the skin and they can also enter the human body when pharmaceuticals or foods are swallowed or eaten. Research suggests that several parabens, have endocrine disrupting effects and they suspected to cause various health effects. Thus, there is an urgent need for the development of novel bioanalytical methods for the biomonitoring of these compounds.
In this study a novel fabric phase sorptive extraction-high performance liquid chromatography method (FPSE-HPLC) has been developed and validated for the quantification of parabens in human plasma. FPSE utilizes a natural or synthetic permeable and flexible fabric substrate chemically coated with a sol-gel organic-inorganic hybrid sorbent in the form of ultra-thin coating. Chromatographic separation was performed on a Spherisorb ODS1 C18 analytical column with isocratic elution using acetonitrile as solvent A and 49.2 mM ammonium formate buffer as solvent B at a flow rate of 0,25 mL min-1. The assay was linear over a concentration range of 20 to 500 ng mL-1 for all the analytes. A run time of less than 30 min for each sample made it possible to analyze many biological samples per day.
Main subject category:
Other subject categories:
Health Sciences
HPLC, Bioanalysis, Human plasma, Parabens, FPSE
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