Unit:
Faculty of MedicineLibrary of the School of Health Sciences
Dissertation committee:
Δημήτριος Δουγένης, Καθηγητής, Τμήμα Ιατρικής, ΕΚΠΑ
Κωνσταντίνος Αναγνωστόπουλος, Καθηγητής, Τμήμα Ιατρικής, ΕΚΠΑ
Ευστράτιος Πατσούρης, Καθηγητής, Τμήμα Ιατρικής, Καθηγητής ΕΚΠΑ
Ιωάννης Ρίζος, Καθηγητής, Τμήμα Ιατρικής, ΕΚΠΑ
Ιωάννης Κακίσης, Καθηγητής, Τμήμα Ιατρικής, ΕΚΠΑ
Ιωάννης Τουμπούλης, Επίκουρος Καθηγητής, Τμήμα Ιατρικής, ΕΚΠΑ
Ανδρέας Λάζαρης, Αναπληρωτής Καθηγητής, Τμήμα Ιατρικής, ΕΚΠΑ
Original Title:
Πειραματικό μοντέλο ισχαιμικής βλάβης νωτιαίου μυελού και καθυστερημένης νευρολογικής βλάβης
Translated title:
Experimental model of ischemic spinal cord injury and delayed neurological injury
Summary:
Introduction:
Potassium adenosine triphosphate (KATP) channel openers have been involved in the enhancement of ischemic tolerance in various tissues. The purpose of the present study is to evaluate the effects of aprikalim, a specific KATP channel opener, on spinal cord ischemic injury.
Materials and methods:
Fifty-four rabbits were randomly assigned to three groups: group 1 (n=18, sham operation), group 2 (n=18, 30 min of normothermic aortic cross-clamping) and group 3 (n=18, aprikalim 100 mg/kg was administered 15 min before 30 min of normothermic aortic cross-clamping). Neurologic evaluation was performed according to the modified Tarlov scale. Six animals from each group were sacrificed at 24, 48 and 168 h postoperatively. The lumbar spinal cords were harvested and examined histologically. The motor neurons were counted and the histologic lesions were scored (0-3, 3: normal).
Results:
Group 3 (aprikalim group) had better Tarlov scores compared to group 2 at all-time points (P < 0.025). The histologic changes were proportional to the Tarlov scores and group 3 had better functional outcome as compared to group 2 at 168 h (number of neurons: 21.2 4.9 vs. 8.0 ± 2.7, P < 0.001 and histologic score: 1.67 ± 1.03 vs. 0.50 ± 0.55, P=0.03). Although aprikalim exhibited improved effect on clinical and histologic neurologic outcome when compared to normothermic spinal cord ischemia, animals in group 3 had worse Tarlov score, reduced number of motor neurons and worse histologic score when compared to group 1 (sham operation) at 168 h (P=0.003, P=0.001 and P=0.019 respectively).
Conclusions:
Aprikalim reduces the severity of spinal cord ischemic injury in a rabbit model of spinal cord ischemia.
Main subject category:
Health Sciences
Keywords:
Aprikalim, K atp channel, openers, Ischemic spinal cord injury protection, Delayed paraplegia, Aortic occlusion, Thoracoabdominal aneurysms, Expression of nitric oxide synthetases, Expression of apoptosis inducing factor, Apoptosis, Neuronal death, Tarlov score
Number of references:
347
