Morphological, biochemical and molecular study o f the beneficial effect of molecules U‐74389G (Lazaroid), Erythropoietin, Sildenafil and Octreotide, in the preservation of kidney graft for transplantation

Doctoral Dissertation uoadl:2943784 77 Read counter

Unit:
Faculty of Medicine
Library of the School of Health Sciences
Deposit date:
2021-05-07
Year:
2021
Author:
Kratiras Zisis
Dissertation committee:
Μιχαήλ Χρυσοφός Καθηγητής, Ιατρική Σχολή, ΕΚΠΑ
Γεώργιος Ζωγράφος, Καθηγητής , Ιατρική Σχολή, ΕΚΠΑ
Απόστολος Παπαλόης, Διευθυντής Ερευνητικού Εργαστηρίου ΕΛΠΕΝ
Χρήστος Αλαμανής, Καθηγητής, Ιατρική Σχολή, ΕΚΠΑ
Κωνσταντίνος Στραβοδήμος, Καθηγητής, Ιατρική Σχολή, ΕΚΠΑ
Ιωάννης Βαρκαράκης, Καθηγητής, Ιατρική Σχολή, ΕΚΠΑ
Νικόλαος Αρκαδόπουλος, Καθηγητής, Ιατρική Σχολή, ΕΚΠΑ
Original Title:
Μορφολογική βιοχημική και μοριακή μελέτη της προστατευτικής δράσης των μορίων U-74389G (Λαζαροειδές), της Ερυθροποιητίνης, της Σιλδεναφίλης και της Οκτρεοτίδης στην συντήρηση νεφρών για μεταμόσχευση
Languages:
Greek
Translated title:
Morphological, biochemical and molecular study o f the beneficial effect of molecules U‐74389G (Lazaroid), Erythropoietin, Sildenafil and Octreotide, in the preservation of kidney graft for transplantation
Summary:
One of the greatest challenges in the field of organ transplantation is the effective organ preservation during the time window from donor site to the recipient. The ischaemia/reperfusion damage after the retrieval/cold storage remains a considerable risk factor for impaired function and potential failure of the grafted organ. Substantial efforts have been undertaken to improve the quality of the kidney grafts, minimizing the IRI.
We studied four different pharmaceutical agents (erythropoietin, sildenafil, lazaroid, octreotide) either as enrichment of the University of Wisconsin solution and or as intravenous pretreatment (administered 1 hour before the graft harvesting). We conducted a double-blind, control group animal study including 56 Landrace/ Large-White pigs (male sex), (age>10 weeks, average weight 22± 2kg), divided in five groups of 6 pigs each. Each group received accordingly one of the four agents mentioned above as iv pretreatment and then as enrichment of the U.W solution. We studied the organs at 3 different time-points (time-point of organ retrieval, 8 and 24 hours post organ harvesting) conducting blood serum analysis, histopathological analysis and oxidative stress assessment.
EPO, Sildenafil and Lazaroid providing encouraging results regarding the 4 histopoathology parameters that were assessed. There was a tendency for improvement, although the results failed to reach statistical significance. Pharmacologically modified preservation solutions, or pretreatment medication appear to be the next step in kidney transplantation in order to reduce the IRI and improve the graft quality.
Main subject category:
Health Sciences
Keywords:
Kidney transplantation, Cold storage, Erythropoietin, Sildenafil, Octreotide, Lazaroid
Index:
No
Number of index pages:
0
Contains images:
Yes
Number of references:
193
Number of pages:
239
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