Unit:
Κατεύθυνση Επιστήμη Πολυμερών και Εφαρμογές της στη ΒιομηχανίαLibrary of the School of Science
Supervisors info:
Ερμόλαος Ιατρού, Καθηγητής, Τμήμα Χημείας, ΕΚΠΑ
Αστέριος Πίσπας, Διευθυντής Ερευνών, Ινστιτούτο Θεωρητικής και Φυσικής Χημείας, Εθνικό Ίδρυμα Ερευνών
Original Title:
Διυδρόφιλα Συσταδικά και Τυχαία Συμπολυμερή POEGMA-b-PMETAC και P(OEGMA-co-METAC): Σύνθεση, Χαρακτηρισμός, Αυτοοργάνωση σε Υδατικά Διαλύματα και Σύμπλεξη με DNA
Translated title:
Double-hydrophilic block and random copolymers POEGMA-b-PMETAC and P(OEGMA-co-METAC): Synthesis, Characterization, Self-assembly in aqueous media and DNA complexation
Summary:
Over the past decades, double-hydrophilic copolymers have attracted the scientific interest because of their ability to self-assemble in aqueous solutions. In the present work, we report the synthesis of novel copolymers that can be applied in gene delivery.
The aim of the current research is the synthesis of double-hydrophilic copolymers poly(oligo(ethylene glycol) methyl ether methacrylate)-b-poly([2-(methacryloyloxy)ethyl] trimethylammonium chloride) (POEGMA-b-PMETAC) and poly(oligo(ethylene glycol) methyl ether methacrylate-co-[2-(methacryloyloxy)ethyl] trimethylammonium chloride) (P(OEGMA-co-METAC)) using the RAFT technique. Their molecular characterization was carried out using size exclusion chromatography (SEC) and proton nuclear magnetic resonance (1H-NMR). We also studied the copolymers’ self-assembling behavior in aqueous solutions under the effect of temperature and ionic strength changes using light scattering techniques (DLS, ELS) and fluorescence spectroscopy (FS). The copolymers’ response to physical and chemical parameters contributes to their application as gene vectors. The cationic polyelectrolyte polymers were complexed with DNA molecules and the properties and behavior of the formed complexes were studied.
Main subject category:
Science
Keywords:
Double-hydrophilic copolymers, RAFT polymerization, DNA complexation
