Unit:
Κατεύθυνση Στοματολογία (Κλινικές Ειδικεύσεις)Βιβλιοθήκη Οδοντιατρικής
Author:
Thermos Grigorios
Supervisors info:
Πιπέρη Ευαγγελία, Επίκουρη Καθηγήτρια, Τμήμα Οδοντιατρικής, Σχολή Επιστημών Υγείας, ΕΚΠΑ
Νικητάκης Νικόλαος, Καθηγητής, Τμήμα Οδοντιατρικής, Σχολή Επιστημών Υγείας, ΕΚΠΑ
Τόσιος Κωνσταντίνος, Αναπληρωτής Καθηγητής, Τμήμα Οδοντιατρικής, Σχολή Επιστημών Υγείας, ΕΚΠΑ
Original Title:
Συγκριτική μελέτη της ανοσοϊστοχημικής έκφρασης των μορίων BMP4 και FOXN1 σε οδοντογενείς κερατινοκύστεις και ορθοκερατινοποιημένες οδοντογενείς κύστεις
Translated title:
Comparative study of the immunohistochemical expression of BMP4 and FOXN1 in odontogenic keratocysts and orthokeratinized odontogenic cyst
Summary:
Introduction: Odontogenic Keratocyst (OKC) and Orthokeratinized Odontogenic Cyst (OOC) are odontogenic cysts of developmental origin that share common features such as keratinized lining epithelium and histological similarity with the epidermis and cutaneous cystic lesions, albeit showing contrasting biological behavior and recurrence rate. Bone morphogenetic protein 4 (BMP4) plays a critical role in the process of odontogenesis regulating the expression of the Sonic Hedgehog (SHH) protein, a molecule implicated in the pathogenesis of OKC. Furthermore, in various tissues of epithelial origin, mainly the interfollicular epidermis and the hair follicle, BMP4 induces the expression of the transcription factor Forkhead box N1 (FOXN1) which participates in the terminal differentiation of epithelial cells as well as the control of cellular proliferation.
Objectives: To investigate and compare the immunohistochemical expression of BMP4 and FOXN1 in OKCs and OOCs.
Materials and Methods: BMP4 and FOXN1 expression was assessed by immunohistochemistry in 20 non-syndromic and non-recurrent OKCs in comparison to 16 OOCs. All specimens were retrieved from the archives of the Oral Pathology Laboratory of the Department of Oral Medicine and Oral Pathology, National and Kapodistrian University of Athens, Greece.
Results: BMP4 epithelial expression was cytoplasmic and was detected in 7/20 (35%) OKCs compared to 13/16 (81,25%) OOCs and this observed difference was statistically significant (p=0,006). Even though no difference was noted in the expression of BMP4 in the connective tissue of the two lesions (p=0,718), a higher percentage of simultaneous positivity of the protein in the epithelium and connective tissue of OOC was seen, in a statistically significant manner (p=0,02). Regarding epithelial FOXN1 expression, it was detected in 12/16 (75%) of OOC cases versus 6/20 (30%) of OKC cases (p=0,007). The double positive phenotype of BMP4 and FOXN1 was more prevalent in OOCs in comparison to OKCs, both in cases where BMP4 was expressed in the epithelium and in cases where the protein’s expression was found in the connective tissue, with these results being statistically significant (p=0,004)
Conclusion: The higher immunohistochemical expression of both BMP4 and FOXN1 as well as the more prevalent BMP4+ FOXN1+ phenotype in OOC suggest a higher activity of the BMP4-FOXN1 axis in this lesion, a finding that is possibly reflected in the more mature epithelial phenotype of OOC compared to OKC.
Main subject category:
Health Sciences
Keywords:
Odontogenic keratocyst, Epidermis, BMP4, FOXN1, Differentiation
Number of references:
386
