Faculty of Medicine
Library of the School of Health Sciences

2021-07-22

2021

Theodoropoulos Grigorios

Ανδρέας Λάζαρης, Καθηγητής , Ιατρική Σχολή, ΕΚΠΑ
Γεωργία Θωμοπούλου, Αναπλ. Καθηγήτρια, Ιατρική Σχολή , ΕΚΠΑ
Χαρά Γιακοπούλου, Αναπλ. Καθηγήτρια, Ιατρική Σχολή, ΕΚΠΑ
Περικλής Φούκας, Αναπλ. Καθηγητής, Ιατρική Σχολή, ΕΚΠΑ
Στρατηγούλα Σακελλαρίου, Επίκουρη Καθηγήτρια, Ιατρική Σχολή, ΕΚΠΑ
Ειρήνη Θυμαρά, Επίκουρη Καθηγήτρια , Ιατρική Σχολή, ΕΚΠΑ
Γεώργιος Αγρογιάννης, Αναπλ. Καθηγητής, Ιατρική Σχολή, ΕΚΠΑ

Ιστική μελέτη στην επιθηλιο-μεσεγχυματική μετατροπή σε νεφροκυτταρικό καρκίνωμα

Greek

Tissue study in epithelial-mesenchymal transition in renal cell carcinoma

Renal cell carcinoma (RCC) is an aggressive malignancy with increased frequency worldwide. Exposed metastatic potential at the time of diagnosis leads the corresponding patients to poor prognosis and survival rates. Among molecules that are involved in cell to cell epithelial conjunction, E-cadherin is critical acting as oncogenes during carcinogenetic process in RCC. Decreased or loss of its expression levels is a severe event in RCC increasing its aggressiveness. Our aim was to investigate the deregulation of E-cadherin and vimentin in RCC tissues in an objective way. We used one hundred (n=100) archival, formalin-fixed and paraffin-embedded RCC tissue specimens, including 75 histologically confirmed clear cell RCC, 13 papillary RCCs, and 12 chromophobe, respectively. Immunohistochemistry was performed implementing an anti- E-cadherin antibody. Digital image analysis was also applied for measuring the corresponding protein expression levels. E-cadherin protein expression demonstrated a progressive decrease in the examined RCC tissue specimens. Negative or loss of its expression was observed in 52 cases, whereas 48 were characterized by moderate/strong (normal) levels. Concerning histological type, loss of e-cadherin was more prominent in clear cell and also papillary variants (p=0.001). Interestingly, a progressive loss of expression was detected according to Fuhrman’s grade classification (p=0.002) in the corresponding analyzed malignant tissues. Low or loss of vimentin expression was observed in 19 cases (19%), whereas the rest of them (n=81 ;81%) demonstrated moderate or dense immunoexpression. Concerning histological type, loss of vimentin was more prominent in clear cell and also papillary variants (p=0.001). Progressive loss of E-cadherin expression is associated with an aggressive phenotype (dedifferentiation, increased metastatic activity) in RCCs, especially in clear cell and papillary histological sub-types, as it happens also with vimentin aberrant expression. Based on their aberrant protein expression, novel agents have been developed for targeting its deregulation enhancing partially its normal function.

Health Sciences

Epithelial, Mesenchymal, Transition, Renal cell, Carcinoma

No

0

Yes

350

135

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https://pergamos.lib.uoa.gr/uoa/dl/object/2958020