Title:
Self-Assembly of a Model Peptide Incorporating a Hexa-Histidine Sequence Attached to an Oligo-Alanine Sequence, and Binding to Gold NTA/Nickel Nanoparticles
Languages of Item:
English
Abstract:
Amyloid fibrils are formed by a model surfactant-like peptide (Ala)10-(His)6 containing a hexa-histidine tag. This peptide undergoes a remarkable two-step self-assembly process with two distinct critical aggregation concentrations (cac's), probed by fluorescence techniques. A micromolar range cac is ascribed to the formation of prefibrillar structures, whereas a millimolar range cac is associated with the formation of well-defined but more compact fibrils. We examine the labeling of these model tagged amyloid fibrils using Ni-NTA functionalized gold nanoparticles (Nanogold). Successful labeling is demonstrated via electron microscopy imaging. The specificity of tagging does not disrupt the β-sheet structure of the peptide fibrils. Binding of fibrils and Nanogold is found to influence the circular dichroism associated with the gold nanoparticle plasmon absorption band. These results highlight a new approach to the fabrication of functionalized amyloid fibrils and the creation of peptide/nanoparticle hybrid materials. © 2014 American Chemical Society.
Authors:
Ian W. Hamley
Steven Kirkham
Ashkan Dehsorkhi
Valeria Castelletto
Jozef Adamcik
Raffaele Mezzenga
Janne Ruokolainen
Claudia Mazzuca
Emanuela Gatto
Mariano Venanzi
Ernesto Placidi
Panayiotis Bilalis
Hermis Iatrou
Journal:
Biomacromolecules
Publisher:
American Chemical Society (ACS)
Keywords:
Amino acids; Dichroism; Glycoproteins; Gold; Hybrid materials; Metal nanoparticles; Self assembly, Critical aggregation concentration; Fluorescence technique; Functionalized gold nanoparticles; Gold Nanoparticles; Millimolar range; Plasmon absorption bands; Self assembly process; Surfactant-like peptides, Peptides, alanine; amyloid; gold nanoparticle; hexahistidine; metal nanoparticle; nickel nanoparticle; nitrilotriacetic acid; peptide; unclassified drug; gold; histidine; metal nanoparticle; nickel; peptide; polyalanine; polyhistidine, amino acid sequence; Article; circular dichroism; hybrid; labeling index; nanofabrication; particle size; peptide synthesis; protein assembly; surface plasmon resonance; transmission electron microscopy, Gold; Histidine; Metal Nanoparticles; Nickel; Peptides
Main subject category:
Science
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