Title:
Polymersomes from Polypeptide Containing Triblock Co- and Terpolymers for Drug Delivery against Pancreatic Cancer: Asymmetry of the External Hydrophilic Blocks
Languages of Item:
English
Abstract:
Well-defined amphiphilic polymers of the ABA and ABC type are synthesized, where A is poly(L-lysine hydrochloride) (PLL), B is poly(γ-benzyl-(d7) L-glutamate) (PBLG(-d7)), and C is poly(ethylene oxide) (PEO). The two polymers exhibit similar PBLG(-d7) composition, while in the ABC, the volume fraction of PEO block is higher than that of PLL. Both polymers form polymersomes in water. The polymersomes are loaded with doxorubicin or paclitaxel. It is found that in the ABC, due to asymmetry of the two hydrophilic blocks, PEO is always on the outer periphery and the dimensions of the vesicles are smaller. The release of the vesicles is temperature-and pH-dependent. In vivo toxicity tests of the empty vesicles show that they are not toxic. In vitro activity of the loaded vesicles against human pancreatic cancer cell lines reveals comparable activity to Myocet for the ABA loaded with doxorubicin, while lower activity is observed for the ABC. © 2014 Wiley-VCH Verlag GmbH & Co. KGaA.
Authors:
Hermis Iatrou
Konstantinos Dimas
Manos Gkikas
Chrisida Tsimblouli
Sosanna Sofianopoulou
Journal:
Macromolecular Bioscience
Keywords:
Nanotechnology; Polypeptides; Amino acids; Cell culture; Diseases; Drug delivery; Ethylene; Hydrophilicity; Nanoparticles; Polyethylene oxides, Hydrophilic blocks; Pancreatic cancers; Polymersomes; Triblocks; Amphiphilic polymers; l-Lysine hydrochloride; Outer periphery; PH-dependent; Poly (ethylene oxide) (PEO), Nanoparticles; Polymers, amphophile; biomaterial; doxorubicin; nanocarrier; nanoparticle; paclitaxel; poly(ethylene oxide) b poly(gamma benzyl glutamate) b poly(lysine hydrochloride); poly(lysine hydrochloride) b poly(gamma benzyl (d7) glutamate) b poly(lysine hydrochloride); polymer; unclassified drug; doxorubicin; paclitaxel; polymer; polypeptide; antineoplastic agent; antineoplastic antibiotic; doxorubicin; drug carrier; macrogol derivative; paclitaxel; poly-gamma-benzyl-L-glutamate; polyglutamic acid; polylysine, adenocarcinoma cell line; animal experiment; Article; controlled drug release; cytotoxicity; electrophoretic mobility; human; human cell; hydrophilicity; hydrophobicity; hypoactivity; loading drug dose; male; molecular weight; mouse; nanoencapsulation; nonhuman; pancreas cancer; pancreatic cancer cell line; pH; polymerization; temperature dependence; toxicity testing; zeta potential; drug delivery system; drug solubility; human cell culture; in vitro study; in vivo study; physical chemistry; temperature; analogs and derivatives; chemistry; drug screening; metabolism; Pancreatic Neoplasms; pathology; synthesis; tumor cell line, Antibiotics, Antineoplastic; Antineoplastic Agents, Phytogenic; Cell Line, Tumor; Doxorubicin; Drug Carriers; Drug Screening Assays, Antitumor; Humans; Nanoparticles; Paclitaxel; Pancreatic Neoplasms; Polyethylene Glycols; Polyglutamic Acid; Polylysine
Main subject category:
Science
Official URL (Publisher):
DOI:
10.1002/mabi.201400137
