Unit:
Κατεύθυνση Ραδιοφαρμακευτική ΧημείαLibrary of the School of Science
Author:
Apostolopoulou Adamantia
Supervisors info:
ΑΡΙΣΤΕΙΔΗΣ ΧΙΩΤΕΛΛΗΣ-ΕΡΕΥΝΗΤΗΣ Γ'-ΕΚΕΦΕ "ΔΗΜΟΚΡΙΤΟΣ"
ΙΩΑΝΝΗΣ ΠΙΡΜΕΤΤΗΣ-ΕΡΕΥΝΗΤΗΣ Α'-ΕΚΕΦΕ "ΔΗΜΟΚΡΙΤΟΣ"
ΑΝΔΡΕΑΣ ΤΣΟΤΙΝΗΣ-ΚΑΘΗΓΗΤΗΣ-ΤΜΗΜΑ ΦΑΡΜΑΚΕΥΤΙΚΗΣ ΕΚΠΑ
Original Title:
ΡΑΔΙΟΕΠΙΣΗΜΑΝΣΕΙΣ ΝΑΝΟΣΩΜΑΤΙΔΙΩΝ ΧΡΥΣΟΥ ΜΕ 99mΤΕΧΝΗΤΙΟ ΓΙΑ ΑΠΕΙΚΟΝΙΣΗ SPECT
Translated title:
RADIOLABELING OF GOLD NANOPARTICLES WITH 99mTECHNETIUM FOR SPECT IMAGING
Summary:
Radiolabeled Gold Nanoparticles (AuNPs) have been widely used for cancer diagnosis and therapy over the last decades. In this study we focus on the development and in vitro evaluation of four new Au nanoconjugates radiolabeled with Technetium-99m (99mTc) via thiol-bearing ligands attached to the NP surface. More specifically, AuNPs of two different sizes (2 nm and 20 nm, referred to as Au(2) and Au(20) respectively) were functionalized with two bifunctional thiol ligands (referred to as L1H and L2H). In vitro cytotoxicity was assessed in 4T1 murine mammary cancer cells. The AuNPs were successfully radiolabeled with 99mTc-carbonyls at high radiochemical purity (> 95%) and showed excellent in vitro stability in competition studies with cysteine and histidine. Moreover, lipophilicity studies were performed in order to determine the lipophilicity of the radiolabeled conjugates, while a hemolysis assay was performed to investigate the biocompatibility of the bare and functionalized AuNPs. We have shown that the functionalized AuNPs developed in this study lead to stable radiolabeled nanoconstructs with the potential to be applied in multimodality imaging or for in vivo tracking of drug-carrying AuNPs.
Main subject category:
Science
Other subject categories:
Health Sciences
Keywords:
radiolabeling, nanoparticles, technetium, cancer
