Supervisors info:
Βερτζώνη Μαρία, Επίκουρη Καθηγήτρια, Τμήμα Φαρμακευτικής, ΕΚΠΑ (επιβλέπουσα)
Ρέππας Χρήστος, Καθηγητής, Τμήμα Φαρμακευτικής, ΕΚΠΑ.
Βλάχου Μαριλένα, Αναπληρώτρια Καθηγήτρια, Τμήμα Φαρμακευτικής, ΕΚΠΑ.
Summary:
Aim: Τhe present study had two objectives. Firstly, evaluate the bacterial degradation of metronidazole and olsalazine in fecal material of elderly subjects (age> 65 years). Data were collected with previously collected data from another 6 elderly subject (Gkolfi 2017). The second objective, was the impact of ageing on drug bacterial degradation. Data from 12 elderly subjects (n=12, 65-91 y, this study, Gkolfi 2017) was evaluated vs data collected from pediatric populations (n=12, 3-8,5 y, Romanos 2017, Romanos 2019) and data collected from adults (n=12, 19-55 y, Karatza et al. 2016, Gkolfi 2020).
Methods: Stools of six elderly people (age range 70-86 years) were collected upon approval by the Bioethical Committee of the Department of Pharmacy. Fecal material was prepared under anaerobic conditions, (10% Η2, 10% CO2, 80% N2, 37οC, relative humidity 70%), after diluting with saline (0.9% sodium chloride) in a ratio of 1:3.8 w/v. The ex vivo degradation kinetic profile of metronidazole and olsalazine was assessed in fecal material under anaerobic conditions.
Results: The mean bacterial degradation rate constant (standard deviation) (n=6) of metronidazole in the fecal material of elderly subjects was 0,169 (0,070) min-1, while the corresponding value for olsalazine was 0,042 (0,014) min-1. There is no statistically significant difference from previously collected data in elderly subjects (n=6) 0,093 (0,79) min-1and 0,037(0,019) min-, respectively (Gkolfi 2017). Based on data collected in 12 elderly subjects (this study, Gkolfi 2017), 12 pediatric populations (Romanos 2017, Romanos 2019) and 12 adults (Karatza 2016, Gkolfi 2020). The impact of ageing on bacterial degradation of metronidazole and olsalazine in fecal material is not statistically significant (Analysis of variance, P= 0,430) and P=0,509 respectively).
Conclusions: Data collected in fecal materials prepared for the needs of the present study did not show statistically significant differences from data collected previously. with similar methodology by other volunteers for the needs of previous study (Gkolfi 2017). Based on data collected in this and previous studies, the impact of ageing on the bacterial degradation of metronidazole and olsalazine is not significant. However, the power of the applied of statistical hypothesis test was low and this conclusion is reported cautiously.
Keywords:
ageing, microbiome, elderly, bacterial degradation, metronidazole, olsalazine ,azoreductase, nitroreductase
