Unit:
Κατεύθυνση Μικροβιακή ΒιοτεχνολογίαLibrary of the School of Science
Author:
Kaltsouda Elisavet
Supervisors info:
Ζαχαρούλα Γκόνου – Ζάγκου, Επίκουρη Καθηγήτρια Τμήματος Βιολογίας ΕΚΠΑ
Original Title:
Απομόνωση ιθαγενών στελεχών εντομοπαθογόνων μυκήτων προς αξιοποίηση τους στον βιολογικό έλεγχο φορέων μυκητιακών ασθενειών σε δασικά δένδρα
Translated title:
Isolation of native strains of entomopathogenic fungi for exploitation in biological control of fungal diseases insect vectors in forest trees
Summary:
It is quite common fungal diseases of forest trees to be disseminated with the aid of insects. Their body morphology as well as their reproduction is well adapted to serve this role. In the present work, isolation of entomopathogenic fungi in pure culture was performed from soil samples. The samples came from sites where trees of Platanus orientalis and Cupressus sempervirens were infected with fungi. Experiments to highlight the most selective medium were preceded. The isolated strains were studied morphologically and identified up to genus level. Most of the strains belong to species of the common genera Beauveria, Paecilomyces and to the quite rare Auxarthron. It is worth mentioning that some strains, which are considered interesting, could not be identified, mainly due to the absence of reproductive organs. Thus, the molecular characterization of the strains was executed, where it was performed with the amplification of the ITS sequence. Finally, contamination experiments were performed on larvae of the insect Τenebrio molitor with the utilization of spore suspension from selected strains.
Main subject category:
Science
Keywords:
entomopathogenic fungi, selective medium, morphological study, molecular characterization
