Title:
Rapid flow injection electrochemical detection of 3,3′,4,4′ tetrachlorobiphenyl using stabilized lipid membranes with incorporated sheep antibody
Languages of Item:
English
Abstract:
An electrochemical biosensor based on a supported polymerized lipid film with incorporated sheep anti-3,3′,4,4′ tetrachlorobiphenyl (PCB congener 77) antibody using flow injection analysis was developed. The polymerized lipid film contained 85% (w/w) dipalmitoylphosphatidylcholine (DPPC) and 15% (w/w) dipalmitoylphosphatidic acid (DPPA), methacrylic acid, ethylene glycol dimethacrylate, AIBN and sheep anti-congener 77 antiserum. Congener 77 was injected into flowing carrier electrolyte and the flow stopped to detect the antigen. These membranes gave only a single transient proportional to log [congener 77] from 10-8 to 10-5 M, with a detection limit of ca. 10-8 M. A membrane containing 35% (w/w) DPPA was used to examine regeneration. The maximum number of cycles was about 5. © 2013 Versita Warsaw and Springer-Verlag Wien.
Authors:
Nikolelis, D.P.
Psaroudakis, N.
Michaloliakos, A.I.
Nikoleli, G.-P.
Scoullos, M.
Journal:
Central European Journal of Chemistry
Official URL (Publisher):
DOI:
10.2478/s11532-012-0157-4
