Supervisors info:
Ελένη Σκαλτσά, Καθηγήτρια, Τμήμα Φαρμακευτικής, ΕΚΠΑ,
Νεκτάριος Αληγιάννης, Αναπληρωτής Καθηγητής Τμήμα Φαρμακευτικής, ΕΚΠΑ,
Προκόπης Μαγιάτης, Αναπληρωτής Καθηγητής Τμήμα Φαρμακευτικής, ΕΚΠΑ
Summary:
Genus Stachys L. consists approximately of 300 species as annual or perennial herbs or small shrubs, which are developed in temperate regions of Mediterranean Sea, Asia, America and southern Africa. In terms of phytochemistry, the plants of the genus are rich in constituents with therapeutic and economic applications, such as terpenes (e.g., triterpenes, diterpenes, iridoids), polyphenols (e.g., flavone derivatives, phenylethanoid glycosides, lignans), phenolic acids and essential oils. Traditionally, plants of this genus have long been used in treatment of genital tumors, sclerosis of the spleen, inflammatory tumors, and cancerous ulcers. In North Greece, Stachys iva Griseb. has been applied against common cold and gastrointestinal disorders. Cultivated populations of Stachys iva Griseb. were provided by the Institute of Breeding and Plant Genetic Resources (Department of Medicinal and Aromatic Plants, Thermi, Thessaloniki). The plant material has been extracted with cyclohexane, dichlromethane: methanol 2:1 and methanol, successively. The dichlromethane: methanol extract has been chosen for phytochemical analyses performed by means of various analytical techniques and NMR spectroscopy. In total, nineteen compounds were isolated and identified, belonging to different phytochemical groups: (i) iridoids, harpagide and 8-acetyl-harpagide; (ii) flavonoids, apigenin, cirsimaritin, penduletin, apigenin 7-O-β-D-glucoside, apigenin 7-(6″-p-coumaroyl)-β-D-glucoside, isoscutellarein 7-O-β-D-allosyl-(1→2)-β-D-glucoside, isoscutellarein 7-O-[6‴-O-acetyl]-β-D-allosyl-(1→2)-β-D-glucoside, isoscutellarein 7-O-[6‴-O-acetyl]-β-D-allosyl-(1→2)-[6″-O-acetyl]-β-D-glucoside, 4′-methyl-isoscutellarein 7-O-[6‴-O-acetyl]-allosyl-(1→2)-glucoside, hypolaetin-7-O-[6‴-O-acetyl]-β-D-allopyranosyl-(1→2)-β-D-glucopyranoside; 4′-methyl-hypolaetin-7-O-[6‴-O-acetyl]-β-D-allopyranosyl-(1→2)-β-D-glucopyranoside, (iii) phenylethanoid glucosides, leucoseptoside A, martynoside, (iv) phenolic acid, 4-hydroxy benzoic acid and (v) polyhydroxy derative, polypotrine. Moreover, the essential oil of S. iva Griseb. has been studied. GC-MS analysis showed that the major chemical classes were diterpenes (27.5%), sesquiterpene hydrocarbons (24.9%) and oxygenated sesquiterpenes (21.5%), while the main constituents were geranyl-α-terpinene (27.5%), caryophyllene oxide (8.6%), α-bisabolol (6.5%), 2-pentadecanone, 6,10,14-trimethyl- (5.8%) and germacrene D (5.3%).
Keywords:
Stachys iva Griseb., cultivated, essential oil, phytochemical investigation, medium polarity extract
