Unit:
Κατεύθυνση Εφαρμογές της Βιολογίας στην ΙατρικήLibrary of the School of Science
Author:
Kotsa Emmanouella
Supervisors info:
Ουρανία Τσιτσιλώνη, Καθηγήτρια Βιολογίας, ΕΚΠΑ
Παναγιώτη Γεωργιάδη, Κύριο Ερευνητή ΙΧΒ-ΕΙΕ
Βασιλική Πλέτσα, Κύρια Ερευνήτρια ΙΧΒ/ΕΙΕ
Original Title:
Επιγενετική της παχυσαρκίας και του διαβήτη τύπου ΙΙ
Translated title:
Epigenetics of obesity and type II diabetes
Summary:
Non-alcoholic fatty liver disease (NAFLD) is the disease characterized by the accumulation of fat in
the liver and is one of the most common causes of chronic liver disease worldwide. It includes a range
of pathological conditions from simple fatty liver to steatohepatitis, fibrosis and cirrhosis. Nonalcoholic fatty liver disease is part of the metabolic syndrome and includes obesity, cholesterol and
triglyceride disorders, type 2 diabetes mellitus (T2DM) and elevated blood pressure. In this work, 50
patients with diagnosed non-alcoholic fatty liver disease at different stages of the disease have been
included. Blood serum was obtained from these patients from which DNA was isolated (cell-free
DNA, n = 50). These samples were used to study, with the Pyrosequencing method, the methylation
of genes related to cellular aging (senescence), namely CDKN1a (p21), CDKN2a (p16) and Rb, and
to investigate whether this differs between the stages of the disease. It was found that in all samples,
the genes appear hypomethylated and that there is a differentiation of methylation between the
different stages of NALFD disease. However, further investigation of the above findings is required.
Main subject category:
Science
Keywords:
NAFLD, CDKN1a, CDKN2a, Rb, epigenetics, methylation, obesity
