Κατεύθυνση Βασική Έρευνα
Library of the School of Health Sciences

2023-03-14

2023

Patakioutis Konstantinos

Αναστάσιος Φιλίππου, Αναπληρωτής Καθηγητής, Ιατρική Σχολή, ΕΚΠΑ
Μιχαήλ Κουτσιλιέρης, Ομότιμος Καθηγητής, Ιατρική Σχολή, ΕΚΠΑ
Αντώνιος Χατζηγεωργίου, Αναπληρωτής Καθηγητής, Ιατρική Σχολή, ΕΚΠΑ

Έκφραση κυτταροκινών μετά από μηχανική φόρτιση μυοβλαστών: ο ρόλος της γήρανσης

Greek

Cytokine expression after mechanical loading of myoblasts: the role of aging

Introduction: Aging is associated with both a decline in muscle functional capacity and a loss of muscle mass accompanied by reduced myogenesis and regenerative capacity in the aged skeletal muscle. However, under regular mechanical stimuli, the impairment of these functions and biological processes can be reversed in the aged muscle, though the effect of mechanical loading on the activation of intracellular pathways and gene expression in aged muscle cells has not been well characterized. The aim of the present study was to investigate the role of aging in the effects of mechanical loading on myogenesis and cytokine expression in skeletal myoblasts.
Methods: Aged C2C12 myoblasts (because of their continuous culture until completion of 80 cell divisions) as well as normal myoblasts were subjected via the Flexcell Tension System to a specific mechanical loading (stretching) protocol, which had the following characteristics: 15% elongation with a frequency of 1Hz for 12 hours. Normal and senescent myoblasts, which had not undergone mechanical loading, were used as a control condition. The levels of mRNA expression of myogenic regulatory factors (Myf5, MyoD, Myogenin) as well as the inflammatory cytokines interleukin (IL)-6 and IL-4, were evaluated. Data are presented as mean ± standard error of the mean (S.E.) and the level of statistical significance was set at p <0.05.
Results: Aged myoblasts showed increased expression of the myogenic factor Myf5 compared to unloaded cells, as well as compared to normal myoblasts, in which mechanical loading led to a decrease in Myf5 expression. Also, reduced expression of MyoD and Myogenin was induced by mechanical loading in both aged and normal myoblasts compared to the corresponding unloaded cells. In addition, the expression levels of IL-6 were found to be reduced in both the loaded aged and normal myoblasts compared to the unloaded cells, while regarding the anti-inflammatory IL-4, the aged myoblasts showed an increased expression compared to the normal cells, in response to their mechanical loading.
Conclusions: The findings of the present study suggest that the specific mechanical loading protocol used induces a differential effect on the expression of myogenic regulatory factors in aged myoblasts while overall, in the aged and control myoblasts, it appears to have an adverse effect on the myogenic processes. Also, this study showed a differential expression of inflammatory factors in aged versus control myoblasts, in response to their mechanical loading. These findings may contribute to the characterization of the molecular responses of aged muscle cells to mechanical stimuli and to the understanding of the molecular mechanisms that regulate mechanotransduction in aged muscle, helping to the development of strategies to prevent and treat muscle dysfunction associated with aging.

Health Sciences

Aging, Cytokines, Inflammation, Interleukins, Mechanical strain, Myoblasts, Myogenesis

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https://pergamos.lib.uoa.gr/uoa/dl/object/3295223