Unit:
Faculty of MedicineLibrary of the School of Health Sciences
Dissertation committee:
Νικόλαος Καβαντζάς, Καθηγητής, Ιατρική Σχολή, ΕΚΠΑ
Ανδρέας Χ. Λάζαρης, Καθηγητής, Ιατρική Σχολή, ΕΚΠΑ
Φραγκίσκη Ανθούλη-Αναγνωστοπούλου, Καθηγήτρια, Βιοϊατρικών Επιστημών Σχολή, ΠΑΔΑ
Κωνσταντίνος Δούρος, Καθηγητής, Ιατρική Σχολή, ΕΚΠΑ
Γεώργιος Αγρογιάννης, Αναπληρωτής Καθηγητής, Ιατρική Σχολή, ΕΚΠΑ
Ειρήνη Θυμαρά, Αναπληρώτρια Καθηγήτρια, Ιατρική Σχολή, ΕΚΠΑ
Στρατηγούλα Σακελλαρίου, Αναπληρώτρια Καθηγήτρια, Ιατρική Σχολή, ΕΚΠΑ
Original Title:
Ιστολογική μελέτη βρογχικών βιοψιών σε παιδιά με εμμένουσα βακτηριακή βρογχίτιδα
Translated title:
Histological study of bronchial biopsies in children with protracted bacterial bronchitis
Summary:
Background: In order to understand the pathogenetic mechanisms of Protracted Bacterial Bronchitis - a syndrome that is among the commonest causes of chronic cough - there is an increasing interest in the study of the immune microenvironment in the bronchial lumen. The aim of this study is the determination of the types and quantity of the inflammatory cells that infiltrate the bronchial epithelium as well as of the thickness of the basement membrane.
Methodology: 97 endobronchial biopsies, obtained from children with chronic (>8 weeks) wet/productive cough, were objected to Hematoxylin and Eosin staining. Using an appropriate image analysis and processing software, we determined the types and the quantity of the inflammatory cells that infiltrated the bronchial epithelium, and the thickness of the basement membrane. The metric data were then subjected to extensive statistical analysis.
Results: According to our results, females had increased levels of eosinophils (p = 0.021) and lymphocytes (p = 0.044) compared to males. Moreover, we found that membrane thickness was negatively correlated with the number of eosinophils (p < 0.0001), neutrophils (p = 0.023) and lymphocytes (p = 0.024). Finally, the pairwise comparisons of the number of eosinophils, neutrophils, lymphocytes and other cell types revealed significant (p < 0.05) positive correlations.
Conclusion: Protracted Bacterial Bronchitis activates pulmonary innate immune pathways. Also, it is accompanied by basement membrane thickening which is a typical characteristic of several respiratory diseases, such as asthma.
Main subject category:
Health Sciences
Keywords:
Bronchitis, Asthma, Protracted bacterial bronchitis, Inflammatory cells, Basement membrane
Number of references:
114
File:
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Ntesou_Despoina_PhD.pdf
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