Unit:
Faculty of MedicineLibrary of the School of Health Sciences
Dissertation committee:
Δημήτριος Μαντάς, Καθηγητής, Iατρική Σχολή, ΕΚΠΑ
Γεράσιμος Τσουρούφλης,Αναπληρωτής Καθηγητής, Iατρική Σχολή, ΕΚΠΑ
Δημήτριος Δημητρούλης, Καθηγητής, Iατρική Σχολή, ΕΚΠΑ
Γρηγόριος Κουράκλης, Ομότιμος Καθηγητής, Iατρική Σχολή, ΕΚΠΑ
Μιχαήλ Κοντός, Αναπληρωτής Καθηγητής, Iατρική Σχολή, ΕΚΠΑ
Αναστάσιος Φιλίππου, Αναπληρωτής Καθηγητής, Iατρική Σχολή, ΕΚΠΑ
Νικόλαος Νικητέας, Καθηγητής,Iατρική Σχολή, ΕΚΠΑ
Original Title:
Η μελέτη του βιορυθμιστικού συστήματος του ινσουλινομιμητικού αυξητικού παράγοντα 1 (IGF-1) στη φυσιολογική επούλωση τραύματος
Translated title:
Study of the bioregulatory system of the insulin-like growth factor 1 (IGF-1) during normal wound healing
Summary:
Αim:
There is increasing evidence to indicate that insulin‑like growth factor (IGF)‑1 plays a crucial role in the regeneration of different tissues following injury. Notably, despite the escalating number of animal studies, studies investigating the role of IGF‑1 in the wound healing process in humans are fewer. In this context, the aim of the present study was to evaluate the variations in the expression IGF‑1 isoforms (IGF1‑Ea, IGF1‑Eb and IGF1‑Ec), as well as its binding protein and receptor (IGF‑BP3 and IGF‑1R) during wound healing in patients.
Μaterial – Methods:
The study population comprised of 21 patients presenting with the first episode of sacrococcygeal pilonidal disease. Samples were obtained during surgery, as well as on days 2, 7 and 14 post‑operatively. The expression levels of IGF‑1 isoforms, as well as that of its binding protein and receptor were evaluated using reverse transcription‑quantitative PCR. Statistical analyses were performed using GraphPad Prism software. The Kruskal‑Wallis test and Dunn's post hoc test were utilized.
Results:
The results revealed a statistically significant difference in the expression of IGF‑BP3 and IGF‑1R during wound healing (P=0.014 and P=0.018, respectively). Specifically, the pairwise post‑hoc Dunn test indicated that IGF‑BP3 expression was significantly decreased on the 2nd post‑operative day compared to the day of surgery, while IGF‑1R expression was significantly increased at 14 days post‑operatively. The expression of the remaining IGF‑1 isoforms was not significantly altered during wound healing.
Conclusion:
IGF‑BP3 and IGF‑1R appear to play a crucial role during the wound healing process, particularly in patients with large open wounds following pilonidal disease treatment. Further studies are warranted to evaluate the exact role, as well as the possible use of these proteins as enhancers of wound healing.
Main subject category:
Health Sciences
Keywords:
Anaplasis, Insulin‑like growth factor 1, Tissue regeneration
Number of references:
103
