Unit:
Faculty of MedicineLibrary of the School of Health Sciences
Author:
Tasopoulos Theodoros
Dissertation committee:
Α. Τσακρής, Καθηγητής, Ιατρική Σχολή, ΕΚΠΑ
Γ. Βρυώνη, Καθηγήτρια, Ιατρική Σχολή, ΕΚΠΑ
Ο. Νάκα, Επίκουρη Καθηγήτρια, Τμήμα Οδοντιατρικής, ΑΠΘ
Κ. Αναστασοπούλου, Αναπληρώτρια Καθηγήτρια, Ιατρική Σχολή, ΕΚΠΑ
Ι. Μελετιάδης, Αναπληρωτής Καθηγητής, Ιατρική Σχολή, ΕΚΠΑ
Β. Κουμάκη, Επίκουρη Καθηγήτρια, Ιατρική Σχολή, ΕΚΠΑ
Γ. Τζανετάκης, Αναπληρωτής Καθηγητής, Οδοντιατρική Σχολή, ΕΚΠΑ
Original Title:
Αποικισμός, προσκόλληση και διείσδυση μυκήτων του γένους Candida σε νεότερα επιστρώματα για οδοντιατρική χρήση
Translated title:
Colonization, adhesion and penetration of Candida Albicans on soft denture lining materials
Summary:
Aim of the study: Knowledge on the quantification of the number as well as the retention and adhesion of Candida albicans blastoconidia on silicone denture liners is limited. Thus, the aim of this in vitro study was to explore the adherence of C. albicans to the surface of five long-term silicone-based soft denture lining materials, using artificial and human saliva.
Materials and methods: A total of 50 specimens (10×10×3 mm) of five long- term resilient liners [Molloplast B (MB); GC Reline Soft (GCRS); Elite Soft Relining (ESR); Tokuyama Sofreliner S (TSS); Ufigel SC (USC)], bonded to a computer-aided design and computer-aided manufacturing denture base, were prepared. The specimens were inoculated and incubated in artificial saliva for 1 and 24 hours with a standardized (2.8×106 cfu/ml) C. albicans suspension and in natural saliva over a period up to 3 weeks. At the end of the incubation period, the specimens were stained with acridine orange and observed using fluorescence microscopy.
Results: After 1 hour, in 24 hours and 3 weeks, MB demonstrated significantly earlier adherence of C. albicans cells and higher change compared to the other chairside materials, where the mean number of cells also increased in the frontal parts after 24 hours. ESR had significant higher mean number of blastoconidia compared to TSS, p=0.006 and GCRS, p < 0.001 materials at 3 weeks. No other differences were observed between the other materials.
Regarding the rate of C. albicans proliferation from 1 to 24 hours immersion in artificial saliva, there was an increase in all materials (Molloplast B, p < 0.001; GC Reline Soft, p=0.220; Elite Soft Relining, p=0.032; Tokuyama Sofreliner S, p=0.001; Ufigel SC, p=0.001). The Ufigel Sc showed a significant 2.5-fold increase at 24 hours and significantly higher change compared to TSS and GCRS materials at 3 weeks immersed in natural saliva.
Regarding the mean number of C. albicans blastoconidia between the two parts at 3 weeks within the materials, the frontal part of MB and ESR demonstrated greater change compared to the lateral part (p=0.024 and p=0.007 respectively), while the frontal part of TSS, p=0.010 indicated lower change compared to the lateral part.
Conclusions: Long-term silicone denture liners accumulated a significant amount of C. albicans blastoconidia in their frontal and lateral part. Their coverage by C. albicans cells increases progressively over time and raises clinical concerns.
Main subject category:
Health Sciences
Keywords:
CAD-CAM denture base, Candida albicans, Fungal growth, Long-term soft denture lining materials
Number of references:
287
