Unit:
Faculty of MedicineLibrary of the School of Health Sciences
Author:
Markopoulos Georgios
Dissertation committee:
Βασίλειος Νικολάου, Αναπληρωτής Καθηγητής, Ιατρική Σχολή, ΕΚΠΑ
Δέσποινα Περρέα, Ομότιμη Καθηγήτρια, Ιατρική Σχολή, ΕΚΠΑ
Δημήτριος Ηλιόπουλος, Καθηγητής ,Ιατρική Σχολή, ΕΚΠΑ
Ευστάθιος Χρονόπουλος, Καθηγητής, Ιατρική Σχολή, ΕΚΠΑ
Όλγα Σαββίδου, Καθηγήτρια, Ιατρική Σχολή, ΕΚΠΑ
Δημήτριος Μαστρόκαλος, Αναπληρωτής Καθηγητής, Ιατρική Σχολή, ΕΚΠΑ
Δημήτριος Παπαδόπουλος, Επίκουρος Καθηγητής, Ιατρική Σχολή, ΕΚΠΑ
Original Title:
Αξιολόγηση οστικού μοσχεύματος εμπλουτισμένου με βιταμίνη D σε προκλητά οστικά ελλείματα κρίσιμου μεγέθους. Πειραματική μελέτη σε χοίρους
Translated title:
Evaluation of vitamin D-enriched bone graft in surgically-induced critical-sized bone defects: An experimental study
Summary:
Introduction
Restoration of bone defects of the craniac vault may require the use of
autografts, allografts, xenografts or synthetic grafts. There are promising data
that vitamin D may play a positive role in bone graft incorporation. The
purpose of the present study is the evaluation of the impact of vitamin D
addition to human-derived bone grafts in healing of critical-sized bone defects
in porcine skull.
Patients & Methods
Four identical critical-sized defects were created in the calvaria of 8
adult Landrace Large White pigs. The 1 st defect was left blank as control, the
2 nd defect was filled with human-derived bone graft, the 3 rd defect was filled
with human-derived bone graft enriched with low concentration of vitamin D (2
mg/ml) and the 4 th defect was filled with human-derived bone graft enriched
with high concentration of vitamin D (10 mg/ml). The animals were sacrificed
after 12 weeks. Harvested tissue specimens were qualitatively evaluated by
histology. New bone formation (bone volume / tissue volume, BV/TV) was
quantitatively measured by histomorphometry.
Results
Signs of bone formation were evident in all bone sockets. Mean values
of BV/TV of the 4 defects were 10.91%, 11.05%, 10.40% and 10.87%
respectively, at 12 weeks. In 5 animals, high concentration of vitamin D
caused a significant improvement of bone formation, in relation to controls. In
3 animals, high concentration of vitamin D was associated with decreased
bone formation compared with controls. No statistical difference was observed
at the graft healing among the 4 graft sites (p > 0.05).
Conclusions
The results of this study have shown that the addition of vitamin D to
human-derived bone grafts does not have a significant effect on bone
formation and graft incorporation in critical-sized bone defects of the porcine
calvaria. Further high quality studies are needed to fully elucidate the role of
vitamin D in bone formation and bone graft union.
Main subject category:
Health Sciences
Keywords:
Vitamin D, Bone grafts, Critical sized defects, Porcine, Calvaria
Number of references:
307
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