Unit:
Speciality PeriodontologyΒιβλιοθήκη Οδοντιατρικής
Supervisors info:
Ιωάννης Καρούσης, Αναπληρωτής Καθηγητής, Τμήμα Οδοντιατρικής, Σχολή Επιστημών Υγείας, ΕΚΠΑ
Γεώργιος Μπομπέτσης, Επίκουρος Καθηγητής, Τμήμα Οδοντιατρικής, Σχολή Επιστημών Υγείας, ΕΚΠΑ
Σπυρίδων Βασιλόπουλος, Επίκουρος Καθηγητής, Τμήμα Οδοντιατρικής, Σχολή Επιστημών Υγείας, ΕΚΠΑ
Original Title:
Effect of low-level laser irradiation (810nm) on the proliferation and differentiation of osteoblast like cells cultured on SLA titanium discs exposed to a peri-implantitis environment
Translated title:
Effect of low-level laser irradiation (810nm) on the proliferation and differentiation of osteoblast like cells cultured on SLA titanium discs exposed to a peri-implantitis environment
Summary:
Background: The goal in the treatment of peri-implantitis is the elimination of
inflammation, as well as re-osseointegration. However, existent data do not favor any
decontamination approach. Thus, the aims of this in vitro study are to assess whether
in a peri-implantitis scenario, air-debriding decontamination method (airflow) with
erythritol powder restores the biocompatibility of titanium discs and furthermore, to
investigate the potent biomodulatory ability of diode laser (810 nm) irradiation to
promote cell proliferation and differentiation of premature osteoblast like cells
(MG63) towards osteocytes.
Methods: The experimental groups consisted of cell cultures seeded on titanium discs
exposed or not in a peri-implantitis environment with or without biomodulation with
laser (810 nm). Infected discs were cleaned with airflow with erythritol powder. Cell
cultures seeded on TCP surfaces with or without biomodulation with laser (810nm)
were used as controls. The study evaluated cell viability, proliferation (MTT assay and
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FDA/PI staining), adhesion (SEM) at 24, 48 and 72 hours and surface roughness
changes (profilometry), as well as the effects of low-level laser irradiation on the
MG63 cells’ genetic profile evaluating ALP, OSC, TGF-b1, Runx-2 and BMP-7
expression at 7, 14 and 21 days.
Results: MTT assay as well as FDA/PI method revealed that cell proliferation did not
show significant differences between sterile and disinfected with airflow titanium
discs at any timepoint. SEM microphotographs at 7 days showed that osteoblast like
cells actively adhered to both sterile and disinfected surfaces, while surface roughness
did not change based on amplitude parameters. Concerning the effect of laser
irradiation (810nm) on the MG63 cells’ genetic profile, the combination of airflow
and LLLT indicated a positive biomodulated effect toward differentiation of
osteoblast like cells.
Conclusion: Osteoblast like cells were able to colonize, proliferate and differentiate in
all tested groups, suggesting a restoration of biocompatibility of infected titanium
discs using airflow. Furthermore, photomodulation (810 nm) may promote the
differentiation of osteoblast like cells cultured in both sterile and disinfected titanium
surfaces.
Main subject category:
Health Sciences
Keywords:
Peri-implantitis, AirFlow, LLLT, Biomodulation, Photomodulation, Reosseointegration
Number of references:
135
File:
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Zampa_Evangelia_Master.pdf
5 MB
File access is restricted only to the intranet of UoA.
