Unit:
Faculty of MedicineLibrary of the School of Health Sciences
Dissertation committee:
Νικόλαος Καβαντζάς, Καθηγητής, Ιατρική Σχολή, ΕΚΠΑ
Ανδρέας X. Λάζαρης, Καθηγητής, Ιατρική Σχολή, ΕΚΠΑ
Ειρήνη Θυμαρά, Αν. Καθηγήτρια, Ιατρική Σχολή, ΕΚΠΑ
Αφροδίτη Νόννη, Καθηγήτρια, Ιατρική Σχολή, ΕΚΠΑ
Χαρά Γακιοπούλου, Καθηγήτρια, Ιατρική Σχολή, ΕΚΠΑ
Λάμπρος Γκουτζάνης, Αν. Καθηγητής, Οδοντιατρική Σχολή, ΕΚΠΑ
Γεώργιος Αγρογιάννης Αν. Καθηγητής, Ιατρική Σχολή, ΕΚΠΑ
Original Title:
Ανοσοϊστοχημική μελέτη δεικτών φλεγμονής σε ακρορριζικές κύστεις
Translated title:
Immunohistochemical study of inflammatory markers in periapical cysts
Summary:
Apical periodontitis – which is related to the inflammation and the destruction of periradicular tissues – is a very common clinical problem. The study of inflammatory markers is essential for the in-depth analysis of this issue. This PhD thesis aims at investigating the immunohistochemical expression of CD34, HSP-70 and Il-6 markers in extracted periapical cysts from male and female patients. The statistical analysis of the results revealed some positive correlations –especially regarding the CD34 levels of protein expression and the MicroVessel Density (MVD) that potentially affect specific clinic-pathological features in these lesions.
More specifically, odontogenic cysts represent a type of lesions with endodontic origin that in some cases mimic even aggressive odontogenic tumors sharing with them similar radiographic features. Periapical cysts (PCs) belong to inflammatory odontogenic cysts sub-category and rarely squamous cell carcinoma arises from their hyperplastic/dysplastic epithelia. Our aim was to explore the impact of cluster differentiation 34 (CD34) protein expression combined with micro vessel density (MVD) on them. Forty-eight (n=48) archival, for¬malin-fixed and paraffin-embedded PC tissue specimens were enrolled in the study. Immunohistochemistry (IHC) was performed in the corresponding tissue sections using an anti- CD34 antibody. CD34 expression levels and also MVD in the examined cases were measured by implementing a digital image analysis protocol. CD34 over expression (moderate to high staining intensity levels) were detected in 29/48 (60.4%) cases, whereas the rest of them (19/48-39.6%) were characterized by low levels of expression. Extended MVD was identified in 26/48 (50.1%) cases correlated with CD34 over expression, epithelial hyperplasia (p-value=0.001), and marginally with inflammatory infiltration level in the examined lesions (p-value=0.056). CD34 over expression combined with increased MVD is associated with a neoplastic-like (hyperplastic) phenotype in PCs as a result of increased neo-angiogenic activity. These histopathological characteristics form potentially and rarely the eligible substrate for squamous cell carcinoma onset in untended cases.
Main subject category:
Health Sciences
Keywords:
Periapical, Odontogenic, Cyst, Microvascular, Density, CD34
Number of references:
355
File:
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Mathiou_Vasiliki_PhD.pdf
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