Unit:
Department of Agricultural Development, Agrofood and Management of Natural ResourcesLibrary of the School of Agricultural Development, Nutrition and Sustainability
Author:
Karagiannaki Elena
Supervisors info:
Αφράτης Νικόλαος, Επίκουρος Καθηγητής, τμήμα Αγροτικής Ανάπτυξης, Αγροδιατροφής και Διαχείρισης Φυσικών Πόρων, ΕΚΠΑ
Παπαγεωργίου Ναυσικά, Επίκουρη Καθηγήτρια, τμήμα Αγροτικής Ανάπτυξης, Αγροδιατροφής και Διαχείρισης Φυσικών Πόρων, ΕΚΠΑ
Τέρπου Αντωνία, Επίκουρη Καθηγήτρια, τμήμα Αγροτικής Ανάπτυξης, Αγροδιατροφής και Διαχείρισης Φυσικών Πόρων, ΕΚΠΑ
Original Title:
Μελέτη της επίδρασης του προβιοτικού Lactobacillus acidophilus στην γονιδιακή έκφραση των μεταλλοπρωτεϊνασών -1 και -9 στον καρκίνο του παχέος εντέρου
Translated title:
Study of the effect of the probiotic Lactobacillus acidophilus on the gene expression of matrix metalloproteinases -1 and -9 in colorectal cancer
Summary:
In the U.S.A, colorectal cancer (CRC) is the third leading cause of cancer death in both men and women, while in Greece it is the second leading cause of death, with a 12% increase between 2011 and 2019. Probiotics have gained increasing medical importance due to the beneficial effect on the human body associated with preventing and supporting the treatment of many chronic diseases, including cancer, without side effects. An important role in the creation and evolution of CRC is played by the interaction of cancer cells with the extracellular matrix (ECM). The proteins that degrades the ECM, such as metalloproteinases (MMPs), play a critical role in cell proliferation, migration and remodeling of the ECM. In CRC, the expression of both MMPs is associated with advanced disease stage and poor prognosis.
In this study, the probiotic Lactobacillus acidophilus was studied to detect its effect on the gene expression of the metalloproteinases MMP-1 and MMP-9 in Caco-2 and HT-29 colon cancer cells. For this purpose, initially, two cancer cell lines were cultured, as well as the probiotic L. acidophilus. Afterwards, the total RNA of the cancer cells was isolated, cDNA was synthesized, and finally real-time PCR was performed to study the gene expression levels. In the Caco2 cancer line, overexpression of both MMPs was observed, while in HT29, overexpression was limited to MMP-1 only, as MMP-9 did not differentiate. This suggests that the presence of the probiotic L. acidophilus may have a negative progression in the course of the disease if administered alone to patients with CRC.
Main subject category:
Science
Keywords:
colorectal cancer, microbiome, extracellular matrix, metalloproteinases
