Unit:
Department of Agricultural Development, Agrofood and Management of Natural ResourcesLibrary of the School of Agricultural Development, Nutrition and Sustainability
Author:
Katechi Kosmina-Zoi
Supervisors info:
Νικόλαος Αφράτης, Επίκουρος καθηγητής, Τμήμα Αγροτικής Ανάπτυξης, Αγροδιατροφής και Διαχείρισης Φυσικών Πόρων, ΕΚΠΑ.
Αντωνία Τέρπου, Επίκουρη καθηγήτρια, Τμήμα Αγροτικής Ανάπτυξης, Αγροδιατροφής και Διαχείρισης Φυσικών Πόρων, ΕΚΠΑ.
Ναυσικά Παπαγεωργίου, Επίκουρη καθηγήτρια, Τμήμα Αγροτικής Ανάπτυξης, Αγροδιατροφής και Διαχείρισης Φυσικών Πόρων, ΕΚΠΑ.
Original Title:
Μελέτη της επίδρασης του προβιοτικού Lactobacillus acidophilus στη γονιδιακή έκφραση του κολλαγόνου τύπου-1 και -4 στον καρκίνο του παχέος εντέρου
Translated title:
Study of the effect of the probiotic Lactobacillus acidophilus on the gene expression of type-1 and -4 collagen in colorectal cancer
Summary:
Probiotics are live microorganisms that belong to the normal human microbiome and have a positive effect on human health. In recent years, the effect of the microbiome on health has been demonstrated and its dysbiosis has been associated with the production of toxins and the development of inflammation which may lead to cancer. For these reasons, some probiotic strains that have been isolated or synthesized in the laboratory are administered to alleviate symptoms or even treat certain diseases, including cancer. Studies have shown that the extracellular space and the macromolecules it contains are influenced by the development and progression of cancer, which makes them useful tools for its prognosis and/or treatment.
In this work, the importance of probiotics in the progression of colorectal cancer was studied. In detail, the probiotic Lactobacillus acidophilus was evaluated in colorectal cancer cells by studying the gene expression of extracellular matrix (ECM) molecules with emphasis on collagen genes. Specifically, two colon cancer cell lines Caco2 and HT-29 were utilized, which were cultured in the presence of condition media derived from the probiotic strain Lactobacillus acidophilus (LA-CM). Total RNA was isolated and gene expression was measured by real-time PCR. In both cancer cell lines collagen type I (Col1A1) and collagen type IV (Col4A1) were analyzed. Col1A1 levels were increased in both HT-29 and Caco2 cancer cell lines, whereas Col4A1 gene expression levels showed an increase only in the Caco2 cell line. In conclusion, it was observed that both type I, fibrillar collagen, and type IV, basement membrane collagen, expression increased due to the presence of LA-CM in colorectal cancer cell culture. Further analysis of the different types of collagens in functional experiments, as well as their analysis at the protein level will provide more reliable conclusions about the importance of these molecules in the progression of the disease.
Main subject category:
Science
Keywords:
colorectal cancer, microbiome, extracellular space, type-1 collagen, type-4 collagen
