Unit:
Faculty of MedicineLibrary of the School of Health Sciences
Author:
Foteinopoulos Panagiotis
Dissertation committee:
Αντσακλής Αριστείδης, Ομότιμος Καθηγητής, Ιατρική Σχολή, ΕΚΠΑ
Παπαντωνίου Νικόλαος, Ομότιμος Καθηγητής, Ιατρική Σχολή, ΕΚΠΑ
Παππά Καλλιόπη, Καθηγήτρια, Ιατρική Σχολή, ΕΚΠΑ
Δασκαλάκης Γεώργιος, Καθηγητή, Ιατρική Σχολή, ΕΚΠΑ
Δημητρακάκης Κωνσταντίνος, Καθηγητής, Ιατρική Σχολή, ΕΚΠΑ
Ντόμαλη Αικατερίνη, Αναπληρώτρια Καθηγήτρια, Ιατρική Σχολή, ΕΚΠΑ
Παπαπαναγιώτου Αγγελική, Αναπληρώτρια Καθηγήτρια, Ιατρική Σχολή, ΕΚΠΑ
Original Title:
Συγκριτική μελέτη της έκφρασης γονιδίων που σχετίζονται με την πολυδυναμικότητα των βλαστικών κυττάρων σε μεσεγχυματικά κύτταρα προερχόμενα από ομφαλίδες φυσιολογικών και παθολογικών εμβρύων με υπολειπόμενη ενδομήτριο ανάπτυξη (Intra-Uterine Growth Restriction, IUGR). Συσχέτιση με κλινικές, υπερηχογραφικές και βιοχημικές παραμέτρους των κυήσεων και επιγενετικός έλεγχος
Translated title:
Comparative study of the expression of genes involved in stem cell pluripotence in mesenchymal stem cells from umbilical cords of normal and IUGR fetuses . Correlation with clinical , sonographic and biochemical variables of gestations and epigenetic control .
Summary:
Background and Objectives: Intrauterine growth restriction (IUGR) is worldwide a public health problem. IUGR-born individuals acquire epigenetic “thrifty-phenotype” gene adaptations, according to the “Barker hypothesis” and are more susceptible to develop metabolic related diseases. Small for Gestational Age (SGA) fetuses consist of a transitional group between IUGR and normally grown fetuses (Appropriate for Gestational Age, AGA).Currently, there is limited information in the literature whether IUGR or SGA status affects the pluripotency of the Umbilical Cord Mesenchymal Stem Cell (UC-MSC) with respect to gene expression at various stages of cell culture.
Materials and Methods: In this study, we investigated stemness marker expression in UC-MSCs derived from twenty-one AGA, IUGR, and SGA fetuses. The relative expression of key genes involved in stemness, pluripotency, and cell proliferation, namely IGF2BP1, CMYC, GLI1, P21, NANOG, OCT4, and SOX2, was assessed in UC-MSCs at passages P0 and P1 using flow cytometry and quantitative PCR.
Results: We found lower median expression levels of IGF2BP1 in IUGR group (P=0.044) and P21 in SGA group (P=0.024) at P0 compared to control group. GLI1 and OCT4 exhibited reduced expression at P1 in the IUGR group (P=0.0434 and P=0.0343 respectively) compared to AGA. NANOG median expression differed statistically between CONTROL and IUGR group in P0 (P=0.034) and P1 (P=0.0205). No significant changes were noted for SOX2 and CMYC median expression among the various groups in P0 and P1.
Conclusion: The comparative analysis of stemness marker expression among UC-MSCs from these three different sources is a novel descriptive aspect of our study, adding a new perspective to the existing literature.
Main subject category:
Health Sciences
Keywords:
Intra-Uterine Growth Restriction , Mesenchymal stem cells , Umbilical cord , Epigenetic control , Gene expression
Number of references:
137
