Unit:
Κατεύθυνση Απομόνωση, Ανάπτυξη, Παραγωγή και Έλεγχος Βιοδραστικών Φυσικών ΠροϊόντωνLibrary of the School of Science
Author:
Champilomatis Ilias
Supervisors info:
Αλέξιος-Λέανδρος Σκαλτσούνης, Καθηγητής, Τμήμα Φαρμακευτικής, ΕΚΠΑ
Original Title:
Fractionation of Cannabis Extracts Using Amberlite XAD-7 Macroporous Resin Coupled with Short-Path-Distillation for the Recovery of Cannabidiol isolate and other Bioactive Constituents
Translated title:
Fractionation of Cannabis Extracts Using Amberlite XAD-7 Macroporous Resin Coupled with Short-Path-Distillation for the Recovery of Cannabidiol isolate and other Bioactive Constituents
Summary:
A pilot-scale protocol was applied for the isolation of high purity cannabidiol (CBD) from hemp extracts. The procedure included extraction of the plant material, winterization using activated carbon for the removal of wax and chlorophylls and decarboxylation of the extract before subjection to short path distillation. This process results in fractions with high CBD content that can produce CBD isolate when treated with frozen n-pentane in a crystallization step.
A different approach was proposed that skips the winterization process, since it causes loss of terpene and cannabinoid content and also makes a difficult procedure technically due to the handling of the activated carbon. Instead of winterization, the use of macroporous resins was introduced that allowed for the extract to be stripped from waxes and terpenes and for a cannabinoid-rich fraction to be produced for use in short path distillation and production of CBD isolate, as in the original protocol. The first fraction containing terpenes (mostly sesquiterpenes) was found to also contain a minor cannabinoid, cannabicitran (CBCT), for which little research has been conducted. The use of macroporous resin allowed for collection of this terpene fraction, while the winterization process did not allow the recovery of the filtrated content.
To choose which resin best adsorbs best CBD from the extract, three different resins were tested in batch experiments, followed by kinetic studies and isotherm tests. Next, the best resin XAD-7 was tested in column mode for the development of an effective, practical and scalable method that was finally implemented in large scale for the creation of a refined large-scale protocol.
Main subject category:
Science
Other subject categories:
Health Sciences
Keywords:
cannabis; macroporous resin; short-path-distillation; pilot-scale; cannabidol; cannabicitran; sesquiterpenoids
File:
File access is restricted until 2027-05-21.
Ilias Champilomatis Master Thesis.pdf
5 MB
File access is restricted until 2027-05-21.
