Unit:
Κατεύθυνση Εφαρμογές της Βιολογίας στην ΙατρικήLibrary of the School of Science
Author:
Antreou Anastasia
Supervisors info:
Ιωάννης Π. Τρουγκάκος, Καθηγητής Βιολογίας Τμήμα Βιολογίας ΕΚΠΑ,
Βασιλική A. Οικονομίδου, Αναπληρώτρια Καθηγήτρια Τμήμα Βιολογίας ΕΚΠΑ,
Χαράλαμπος Ν. Αλεξόπουλος Επίκουρος Καθηγητής Τμήμα Βιολογίας ΕΚΠΑ
Original Title:
Συστημική (systems biology) μελέτη του δικτύου πρωτεόστασης κατά τη γήρανση και σε νόσους του γήρατος στον οργανισμό μοντέλο Drosophila melanogaster.
Translated title:
Systematic study of proteostasis network during aging and senile diseases in the model organism Drosophila melanogaster.
Summary:
In this research was studied the proteostasis network during aging and senile diseases in the model organism Drosophila melanogaster. In particular, we examined the silencing of the mitochondrial protein Opa1 tissue-specifically and examined the activity of the proteasome, lysosomes and ROS levels. In addition, the longevity and locomotion of the insects are analyzed, western immunoblotting for the detection of oxidative load as well as immunolocalization with fluorescence microscopes for the visualization of mitochondria in four tissues (Muscle, Brain, Fat Body and Intestine) and at two different time points (three & seven days old). In addition, it is silenced from embryogenesis and induced at a later stage using the hormone RU486.
When Opa1 is silenced in muscle tissue there is communication between muscle tissue, nerve tissue and adipose tissue
Embryogenesis silencing does not affect lifespan but reduces climbing ability. Silencing at a later stage affects lifespan and reduces climbing ability.
When Opa1 is silenced in the nervous tissue, communication between muscle and nervous tissue is observed. When it occurs from embryogenesis it affects life span and climbing ability. When it occurs at a later stage neither life span nor climbing ability is affected.
During the silencing of Opa1 in adipose tissue it is observed. communication between tissues. When it occurs from embryogenesis the life span is not affected but the ability to climb is affected the same results occur during silencing at a later stage
Main subject category:
Science
Keywords:
ageing, proteasome, lysosome, ROS, mitochondria, Opa1, muscle tissue, nervous tissues, adipose tissues
Number of references:
187
File:
File access is restricted only to the intranet of UoA.
Συστιμική μελέτη του δικτύου πρωτεόστασης κατά τη γήρανση και σε νόσους του γήρατος στον οργανισμό μοντέλο Drosophila melanogaster.pdf
58 MB
File access is restricted only to the intranet of UoA.
