Supervisors info:
Χρήστος Κόκκινος Αναπληρωτής Καθηγητής Τμήμα Χημείας ΕΚΠΑ, (Επιβλέπων)
Αναστάσιος Οικονόμου, Καθηγητής, Τμήμα Χημείας ΕΚΠΑ,
Αθηνά Μάρκου, Επίκουρη Καθηγήτρια, Τμήμα Χημείας ΕΚΠΑ
Summary:
In recent years, with the advancement of science and technology, the detection of various biomarkers related to human health has gained increasingly importance. Biomarkers are a useful tool for the detection of many diseases, such as cancer, cardiovascular diseases, neurological disorders, as well as kidney diseases. Therefore, it is crucial to detect such diseases using fast, simple, and easy techniques. Electrochemical methods employing smart sensors have emerged as one of the most promising approaches.
The aim of this research is the fabrication of 3D printed electrochemical sensor for the determination of biomarkers in biological fluids. More specifically, the target biomarkers were adenine, guanine, and creatinine, using Differential Pulse Voltammetry (DPV). 3D printed carbon black/ polylactic acid sensors were used for the simultaneous determination of adenine and guanine, while 3D printed carbon black/ polylactic acid sensors modified with copper oxide were used for the determination of creatinine. In both cases, the parameters of each voltammetric method were studied and optimized, and then, the 3D printed sensors were successfully applied to the determination of target biomarkers in real samples. The developed methods were straightforward, rapid, environmentally friendly and provided low limits of detection equal to 0.19 μM for the simultaneous determination of adenine and guanine, and 1.5 μM for the determination of creatinine.
Keywords:
3D printed electrochemical sensor, determination of biomarkers in biological fluids, adenine, guanine, creatinine
